An array substrate includes a base and a pixel defining layer and light-emitting layers that are disposed on the base. The pixel defining layer includes defining strips extending in a first direction and defining portions extending in a second direction, the defining strips and the defining portions define openings, defining portions located between two adjacent defining strips are spaced apart in the first direction, a defining portion includes at least two second sub-portions and first sub-portion(s) connected to the two adjacent defining strips through the at least two second sub-portions, thickness of a defining strip is greater than a thickness of a first sub-portion, and the thickness of the first sub-portion is greater than a thickness of a second sub-portion. Portion(s) of a light-emitting layer is disposed in a corresponding opening, and at least two light-emitting layers between the two adjacent defining strips are connected to form a one-piece structure.