- 专利标题: Methods for nucleic acid sequencing by tunneling recognition
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申请号: US18124455申请日: 2023-03-21
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公开(公告)号: US11981963B2公开(公告)日: 2024-05-14
- 发明人: Yann Astier
- 申请人: Roche Sequencing Solutions, Inc.
- 申请人地址: US CA Pleasanton
- 专利权人: Roche Sequencing Solutions, Inc.
- 当前专利权人: Roche Sequencing Solutions, Inc.
- 当前专利权人地址: US CA Pleasanton
- 代理机构: Kilpatrick Townsend and Stockton LLP
- 主分类号: C12Q1/6869
- IPC分类号: C12Q1/6869 ; G01N27/327 ; G01N33/487
摘要:
Methods for analyzing a nucleic acid molecule are described. Methods may include attaching the nucleic acid molecule to a particle having a first characteristic dimension. In addition, methods may include applying an electric field through an aperture to move the particle to the aperture. Also, methods may include applying a voltage across a first electrode and a second electrode. Further, methods may include contacting a portion of the nucleic acid molecule to both the first electrode and the second electrode within the aperture, where the portion may include a nucleotide. In addition, methods may include measuring a current through the first electrode, the portion of the nucleic acid molecule, and the second electrode, where the measured current runs in a direction parallel to a longitudinal axis of the aperture. Also, methods may include identifying the nucleotide of the portion of the nucleic acid molecule based on the current.
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