公开(公告)号：US12129513B1

公开(公告)日：2024-10-29

申请号：US18679053

申请日：2024-05-30

申请人： Roche Sequencing Solutions, Inc.

发明人： Garry P. Nolan

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/686

CPC分类号： C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/686

摘要： Provided herein is a method for barcoding, comprising: (a) obtaining multiple populations of cells or cell organelles in a plurality of first volumes, wherein: i. within each first volume the cells or cell organelles comprise nucleic acid molecules that are associated with a first subcode of a set of first subcodes, and ii. in different first volumes the nucleic acid molecules are associated with different first subcodes of the set of first subcodes, (b) pooling the cells or cell organelles, (c) separating the pooled cells or cell organelles into a plurality of second volumes, and (d) associating the nucleic acid molecules with a set of second subcodes in the second volumes, wherein a plurality of the second volumes each receive a different second subcode. This method produces at least some nucleic acid molecules that comprise a first subcode and a second subcode.

公开(公告)号：US20240327818A1

公开(公告)日：2024-10-03

申请号：US18580558

申请日：2022-07-21

申请人： Roche Sequencing Solutions, Inc.

发明人： Pantea GHEIBI ,  Keynttisha JEFFERSON

IPC分类号： C12N15/10 ,  C12Q1/6806

CPC分类号： C12N15/101 ,  C12Q1/6806

摘要： Aspects provide a method of isolating RNA from a biological sample. The method may include adding the biological sample to a first electrolyte to form a first mixture. The method may include applying a voltage difference between a first electrode and a second electrode. A gel may include a portion of a second electrolyte. The method may include flowing, using the voltage difference, the first subset of RNA molecules into one or more focused zones within the second electrolyte to the second electrode. The method may include separating the second subset of RNA molecules from the first subset. The method may include collecting the first subset of RNA molecules by collecting a second mixture comprising the one or more focused zones. The concentration of the first subset in the second mixture is higher than the concentration of the first subset in the biological sample. Related systems are also described.

公开(公告)号：US20240318228A1

公开(公告)日：2024-09-26

申请号：US18677555

申请日：2024-05-29

申请人： Roche Sequencing Solutions, Inc.

发明人： Garry P. Nolan

IPC分类号： C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/686

CPC分类号： C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/686

摘要： The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.

公开(公告)号：US12077817B2

公开(公告)日：2024-09-03

申请号：US17687003

申请日：2022-03-04

申请人： Roche Sequencing Solutions, Inc.

发明人： Randall Davis ,  Roger Chen

IPC分类号： C12Q1/6869 ,  C12N15/11 ,  G01N33/487 ,  G01N33/543

CPC分类号： C12Q1/6869 ,  C12N15/111 ,  G01N33/48721 ,  G01N33/5432 ,  B01J2219/00313 ,  B01J2219/00653 ,  B01J2219/00734 ,  C12N2310/11 ,  C12N2310/16 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N2320/32 ,  C12Q2565/607 ,  C12Q2565/631 ,  C12Q1/6869 ,  C12Q2565/607 ,  C12Q2565/631

摘要： The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

公开(公告)号：US20240287606A1

公开(公告)日：2024-08-29

申请号：US18475498

申请日：2023-09-27

申请人： Roche Sequencing Solutions, Inc.

发明人： Jan Berka ,  Richard Dannebaum ,  Khai Luong ,  Florian Rubelt ,  Dilduz Telman

IPC分类号： C12Q1/6881

CPC分类号： C12Q1/6881

摘要： The disclosure includes methods and compositions for accurately detecting subject's immune cell repertoire based on sequencing genomic DNA of immune cells.

公开(公告)号：US20240279725A1

公开(公告)日：2024-08-22

申请号：US18004365

申请日：2021-07-07

申请人： Roche Sequencing Solutions, Inc. ,  Kapa Biosystems, Inc.

发明人： Richard Dannebaum ,  Brian Christopher Godwin ,  David L. Penkler ,  Etienne Slabbert ,  Ruben van der Merwe

IPC分类号： C12Q1/6848 ,  C12Q1/6876

CPC分类号： C12Q1/6848 ,  C12Q1/6876

摘要： The present disclosure is directed to compositions, kits, and methods of target enrichment by unidirectional primer extension, whereby the compositions, kits, and methods utilize both poison primers and target capture primers.

公开(公告)号：US20240240240A1

公开(公告)日：2024-07-18

申请号：US18561986

申请日：2022-05-12

申请人： Roche Sequencing Solutions, Inc

发明人： Rui Chen ,  Toumy Guettouche ,  Donald Sharon

IPC分类号： C12Q1/6832 ,  C12Q1/6869 ,  C12Q1/6886

CPC分类号： C12Q1/6832 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156

摘要： The invention includes improved methods and compositions for nucleic acid hybridization wherein the improvement comprises the use of enhancer oligonucleotides. Target enrichment is performed using probe oligonucleotides, wherein each probe oligonucleotide comprising a target-binding region, and a first and a second primer-binding region, and one or more enhancer oligonucleotides capable of hybridizing to at least one of the primer binding regions. The forward and reverse primer binding sites can be universal primer binding sites.

公开(公告)号：US20240209432A1

公开(公告)日：2024-06-27

申请号：US18343470

申请日：2023-06-28

申请人： Roche Molecular Systems, Inc. ,  Roche Sequencing Solutions, Inc.

发明人： Frank BERGMANN ,  Christoph SEIDEL ,  Andrew TRANS ,  Dmitriy GREMYACHINSKIY ,  Hannes KUCHELMEISTER ,  Lars HILLRINGHAUS

IPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H19/20

CPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H19/20 ,  C12Q2525/113 ,  C12Q2525/197 ,  C12Q2525/203 ,  C12Q2525/204 ,  C12Q2565/631

摘要： The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.

公开(公告)号：US20240158780A1

公开(公告)日：2024-05-16

申请号：US18453722

申请日：2023-08-22

申请人： Roche Sequencing Solutions, Inc. ,  Kapa Biosystems, Inc.

发明人： Daniel Burgess ,  Brian Christopher Godwin ,  Alexander Lovejoy ,  Bronwen Miller ,  Jo-Anne Elizabeth Penkler ,  Joseph Platzer

IPC分类号： C12N15/10 ,  C12Q1/6813 ,  C12Q1/6874 ,  C12Q1/6876

CPC分类号： C12N15/1034 ,  C12N15/1072 ,  C12Q1/6813 ,  C12Q1/6874 ,  C12Q1/6876

摘要： The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.

公开(公告)号：US20240149536A1

公开(公告)日：2024-05-09

申请号：US18545879

申请日：2023-12-19

申请人： Roche Sequencing Solutions, Inc.

发明人： Roger J.A. Chen ,  Randy Davis

IPC分类号： B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/487

CPC分类号： B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/48721 ,  B82Y10/00

摘要： Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.