Methods, apparatus, systems for video processing, including video encoding or video decoding are described. One example method includes performing a conversion between a video including a video picture that includes one or more subpictures and a bitstream of the video. The bitstream conforms to a format rule specifying that a first syntax element indicating whether an operation is performed across a boundary of a subpicture in the coded layer video sequence is selectively included responsive to a number of subpictures in the video picture.