The present application discloses a display panel, and a manufacturing method thereof, and a display device, which belong to the field of display technologies. The display panel includes a substrate, and a pixel defining layer and a light-emitting device which are disposed on the substrate. In the light-emitting device, a central region of the light-emitting layer covers a first electrode, and a peripheral region of the light-emitting layer is staggered from the first electrode.