A cabinet with anti-condensation mechanism is used to control opening and closing a cabinet door of the cabinet. The cabinet includes a control module, a temperature control module, and a locking module. The control module senses a cabinet-inside temperature inside the cabinet, and senses cabinet-outside temperature and a cabinet-outside humidity outside the cabinet to generate a dew-point threshold value. The temperature control module is used to adjust the cabinet-inside temperature. The locking module is used to lock the cabinet door or unlock the cabinet door. The control module controls the locking module according to the cabinet-inside temperature and the dew-point threshold value, and controls the temperature control module to adjust the cabinet-inside temperature to be greater than or equal to the dew-point threshold value when the control module receives a trigger signal.