A method of disinfecting an object using cryogenic temperatures, and a cryogenic disinfection device and cryogenic disinfection tunnel for use in such a method are disclosed herein. In various embodiments, one or more chambers are provided, capable of providing an ultralow temperature therein. An object is placed or moved to within the chamber and subjected to ultralow, e.g., cryogenic, temperatures therein for a period of time sufficient to disinfect the object to a desired depth.