Embodiments of the disclosure provide a display substrate and a method for manufacturing the same. The display substrate includes: a base substrate; a thin film transistor including a source-drain metal layer and a first insulating layer; a second insulating layer; a color resist layer; and a third insulating layer. The third insulating layer comprises a first via hole that sequentially penetrates the third insulating layer, the color resist layer and the second insulating layer and thus extends from the third insulating layer to the source-drain metal layer. A sidewall of the first via hole comprises a first portion formed of a material of the second insulating layer, a second portion formed of a material of the color resist layer, and a third portion formed of a material of the third insulating layer, the second portion is between the first portion and the third portion.