A biosensor using an exceptional point is disclosed. A biosensor according to one embodiment of the present disclosure includes: a biosensing unit configured to output wavelength-separated optical signals from destruction of an exceptional point resulting from attachment of biomolecules; a detection unit configured to convert the wavelength-separated optical signals into wavelength-separated electrical signals; an analysis unit configured to measure a beat frequency resulting from the wavelength-separated electrical signals; and a determination unit configured to determine a wavelength difference resulting from the beat frequency, thereby determining the amount of the biomolecules therefrom.