A display substrate has sub-pixels. Each column of sub-pixels includes first and second sub-pixels alternately arranged. The display substrate includes a base, a second source-drain metal layer including first and second connection portions, and a first source-drain metal layer including first and second data lines alternately arranged. A pixel driving circuit of each first sub-pixel is connected to a second end of a first connection portion, and a first end there is connected to a corresponding first data line. A pixel driving circuit of each second sub-pixel is connected to a second end of a second connection portion, and a first end thereof is connected to a corresponding second data line. In a same column of sub-pixels, an extension direction of a first line connecting a second end of a first connection portion and a second end of a second connection portion is substantially parallel to a second direction.