A generating device for simulating a particulate environment for an experimental animal includes an experimental chamber. An ultrasonic atomization device is installed at the bottom adjacent to the center of the experimental chamber. The upper side of the ultrasonic atomization device is connected to a guide device for guiding particles. The guide device includes an air seal cover, and a micro fan is installed inside the air seal cover to drive the movement of particles generated in the ultrasonic atomization device. Air guide pipes are fixedly connected to a side wall of the air seal cover. The generating device has good sealing performance, and can generate a variety of particle simulation environments to accurately and effectively simulate the biological effect of particles in the animal body. The generating device can improve the particle simulation environment, reduce the deviation of experimental data, and ensure the accuracy of the overall experimental data.