There is provided a display substrate including a base and a pixel circuit layer on the base, the pixel circuit layer includes pixel driving circuits arranged in an array along a first direction and a second direction. The base has recesses, each of which extends in the first direction and has a bottom surface and a side surface, a surface of the base includes the bottom surface and the side surface of each recesses, and a top surface between adjacent recesses, the bottom surface of each recess is substantially parallel to the top surface between adjacent recesses, the side surface of each recesses is at a first angle to the bottom surface of the recess and the top surface between adjacent recesses, and a portion of each pixel driving circuits is formed on the side surface of one recess. A method for preparing a display substrate is further provided.