A display substrate and a display device, the display substrate includes a base substrate, a pixel driving circuit layer, a first planarization layer, a first metal layer, a second planarization layer, a plurality of first electrodes and a pixel definition layer; the pixel driving circuit layer includes a plurality of pixel driving circuits, the first planarization layer includes a plurality of first vias respectively exposing output terminals of the pixel driving circuits, the first metal layer includes a plurality of data lines extending in a first direction, the pixel definition layer includes a plurality of first definition walls extending in the first direction and a plurality of second definition walls extending in a second direction, and an orthographic projection of at least part of the data lines on the base substrate respectively overlaps with orthographic projections of the plurality of first definition walls on the base substrate.