Invention Grant
- Patent Title: Cloning and expression system for T-cell receptors
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Application No.: US15780938Application Date: 2016-12-02
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Publication No.: US12297427B2Publication Date: 2025-05-13
- Inventor: Paul Thomas , Xizhi Guo , Pradyot Dash
- Applicant: St. Jude Children's Research Hospital, Inc.
- Applicant Address: US TN Memphis
- Assignee: St. Jude Children's Research Hospital, Inc.
- Current Assignee: St. Jude Children's Research Hospital, Inc.
- Current Assignee Address: US TN Memphis
- Agency: Troutman Pepper Locke LLP
- International Application: PCT/US2016/064735 WO 20161202
- International Announcement: WO2017/096239 WO 20170608
- Main IPC: C40B50/06
- IPC: C40B50/06 ; C07K14/725 ; C12N15/10 ; C12N15/66
Abstract:
The invention provides a method for rapid cloning of T-cell receptors (TCRs) (e.g., paired αβ and γδ TCR chains) and B-cell receptors (BCRs) (e.g. paired IgH or IgK or Igλ) from single cells by CDR3 substitution using single cell PCR products and Gibson Assembly techniques and a pre-generated TCR (or BCR) library in an expression vector.
Public/Granted literature
- US20190040381A1 CLONING AND EXPRESSION SYSTEM FOR T-CELL RECEPTORS Public/Granted day:2019-02-07
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