The present invention provides microfluidic chromatography devices for separating an analyte from a sample solution, and methods for producing and using the same. In particular, the present invention relates to microfluidic devices which comprise a microfabricated flow channel and a material delivery system for transporting a material through the flow channel. The flow channel comprises a chromatography column portion having a solid stationary phase which is capable of separating at least a portion of the analyte from the sample solution.