A device for collecting, testing and transporting a liquid specimen of urine or the like incorporates a cup or container and a cooperating lid which becomes sealed about its upper rim when the lid is mated with the specimen-containing cup. An upwardly open subchamber of defined volume, formed in the bottom wall of the cup, is sealed by engagement with a downwardly extending hollow plug portion of the lid that has a bottom wall containing a central aperture closed by a frangible sheet material seal. When initial testing is to be conducted, an elongated assay cartridge is inserted vertically downward through the hollow plug. The bottom end splits open the frangible seal and enters into the subchamber containing the defined volume of the liquid sample, which becomes totally absorbed by a pair of elongated nitrocellulose strips, along which the liquid moves by capillary action to effect the assay tests.