Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays

Invention Application

US20030119005A1 Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays 失效

Title translation: 核酸的后合成标记，使用合成后标记的核酸，单核苷酸多态性检测以及相关化合物和微阵列进行测定

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Patent Title: Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays
Patent Title (中): 核酸的后合成标记，使用合成后标记的核酸，单核苷酸多态性检测以及相关化合物和微阵列进行测定
Application No.: US10022014

Application Date: 2001-12-14
Publication No.: US20030119005A1

Publication Date: 2003-06-26
Inventor: Charles K. Brush , Vineet Gupta
Applicant: MOTOROLA, INC.
Applicant Address: null
Assignee: MOTOROLA, INC.
Current Assignee: MOTOROLA, INC.
Current Assignee Address: null
Main IPC: C12Q001/68
IPC: C12Q001/68 ; C12M001/34

Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays

Abstract:

An assay is provided for nucleic acids that can be post-synthetically labeled, wherein modified nucleoside triphosphates are used that are more efficiently and specifically incorporated during nucleic acid synthesis than labeled nucleoside triphosphates. In a preferred embodiment, nucleoside null-thiotriphosphates are utilized. Maleimide or iodoacetamide conjugating moieties can be attached post-synthetically. The conjugating moieties may include a reporter group. Also disclosed are new methods for detecting single nucleotide polymorphism.

Abstract(Chinese):

提供了可以进行后合成标记的核酸的测定法，其中使用在核酸合成中比标记的核苷三磷酸更有效和特异性并入的修饰的核苷三磷酸。在优选的实施方案中，使用核苷α-硫代三磷酸酯。马来酰亚胺或碘乙酰胺共轭部分可以在合成后连接。缀合部分可以包括报告基团。还公开了用于检测单核苷酸多态性的新方法。

Public/Granted literature

US07223538B2 Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays Public/Granted day:2007-05-29

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