公开(公告)号：US20040265956A1

公开(公告)日：2004-12-30

申请号：US10701923

申请日：2003-11-06

发明人： Rie Takikawa ,  Akira Imaizumi ,  Kazuhiko Matsui ,  Hiroyuki Kojima

IPC分类号： C12P013/04 ,  C12P013/08 ,  C12Q001/68 ,  C07H021/04 ,  C12N015/74

CPC分类号： C12P13/08

摘要： The present invention describes a method for producing a target substance using a microorganism comprising culturing an Escherichia bacterium in a medium to produce and accumulate the target substance in the medium or the bacterium, and collecting the target substance. More specifically, the bacterium of the present invention can be a strain in which the fis gene on bacterial chromosome is disrupted so that the FIS protein does not function normally in the bacterium.

摘要翻译： 本发明描述了使用微生物制备目标物质的方法，所述微生物包括在培养基中培养大肠杆菌细菌以在培养基或细菌中产生和积累目标物质，并收集目标物质。 更具体地说，本发明的细菌可以是细菌染色体上的fis基因被破坏，使得FIS蛋白在细菌中不起作用的菌株。

公开(公告)号：US20040265934A1

公开(公告)日：2004-12-30

申请号：US10786076

申请日：2004-02-26

申请人： Dako A/S

发明人： Henrik Stender ,  Kaare Lund ,  Tina Anderson Mollerup

IPC分类号： C12Q001/68 ,  G01N033/554 ,  G01N033/569

CPC分类号： C07K14/003 ,  C12Q1/689

摘要： Novel hybridisation assay probes and mixtures of such probes for detecting a target sequence of one or mycobacteria optionally present in a sample. The probes may suitable be directed to target sequences of mycobacterial rDNA, precursor rRNA, or rRNA, said probes being capable of forming detectable hybrids. The probes are in particular directed to mycobacterial rDNA, to precursor rRNA, or to 23S, 16S or 5S rRNA The probes are useful for detecting the organisms in test samples such as sputum, laryngeal swabs, gastric lavage, bronchial washings, biopsies, aspirates, expectorates, body fluids (spinal, pleural, pericardial, synovial, blood, pus, bone marrow), urine, tissue sections as well as food samples, soil, air and water samples, and cultures thereof.

摘要翻译： 新型杂交测定探针和这种探针的混合物，用于检测任选存在于样品中的一种或分枝杆菌的靶序列。 探针可以适用于分枝杆菌rDNA，前体rRNA或rRNA的靶序列，所述探针能够形成可检测的杂交体。 探针特别针对分枝杆菌rDNA，前体rRNA或23S，16S或5S rRNA。探针可用于检测测试样品中的生物体，如痰液，喉拭子，胃灌洗液，支气管洗涤液，活检组织，抽吸液， 痰液，体液（脊髓，胸膜，心包，滑液，血液，脓液，骨髓），尿液，组织切片以及食物样品，土壤，空气和水样品及其培养物。

公开(公告)号：US20040265892A1

公开(公告)日：2004-12-30

申请号：US10891161

申请日：2004-07-13

发明人： Carl T. Wittwer ,  David R. Hillyard

IPC分类号： C12Q001/68 ,  C12P019/34 ,  C12M001/34

CPC分类号： B01L9/065 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0838 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2300/1872 ,  B01L2300/1883 ,  G01N30/02 ,  G01N30/06 ,  G01N27/447 ,  B01D15/34

摘要： A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Once a heating cycle is completed, the controller opens a door to the chamber for venting hot air out and cool ambient air is moved in. Temperature versus time profiles corresponding to optimum denaturation, annealing and elongation temperatures for amplification of DNA are achieved by the present invention.

摘要翻译： 公开了一种热循环方法和装置。 该装置包括样品室，其温度可以在进行许多生物程序（例如DNA聚合酶链式反应）所需的温度范围内快速和准确地调制。 将生物样品置于玻璃微毛细管中，然后置于样品室内。 可编程控制器调节样品室内样品的温度。 一旦加热循环完成，控制器打开一个门，以便排出热空气，冷却环境空气进入。对于扩增DNA的最佳变性，退火和伸长温度对应的温度对时间曲线由本发明实现 发明。

公开(公告)号：US20040265887A1

公开(公告)日：2004-12-30

申请号：US10870577

申请日：2004-06-16

申请人： THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE

发明人： James G. Herman ,  Stephen B. Baylin ,  Manel Esteller

IPC分类号： C12Q001/68 ,  C12P019/34

CPC分类号： C07K16/40 ,  C12Q1/683 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/154 ,  Y10S435/975 ,  C12Q2523/125

摘要： The present invention provides methods relating to chemotherapeutic treatment of a cell proliferative disorder. In particular, a method is provided for predicting the clinical response to certain types of chemotherapeutic agents. Alkylating agents, used for the treatment of certain types of tumors including tumors of the nervous system and lymph system, are efficacious agents when the damage they do to tumor cell DNA is not repaired by cellular DNA repair mechanisms. The present invention provides a method for determining the activity of a gene encoding a DNA repair enzyme, thus providing a prediction of the clinical response to alkylating agents.

公开(公告)号：US20040265885A1

公开(公告)日：2004-12-30

申请号：US10863999

申请日：2004-06-09

申请人： Aventis Pharma Deutschland GmbH

发明人： Eugen Uhlmann ,  Gerhard Breipohl ,  David William Will

IPC分类号： C12Q001/68 ,  C07K014/00 ,  A61K048/00

CPC分类号： C07H21/00

摘要： The present invention relates to PNA derivatives which carry, at the N terminus of the PNA backbone, a phosphoryl radical. The phosphoryl radical can be, for example, a phosphate radical, or a substituted phosphoryl radical, with substituted phosphoryl derivatives carrying, where appropriate, one or more labeling groups, groups for crosslinking, groups which promote intracellular uptake, or groups which increase the binding affinity of the PNA derivative for nucleic acids. The invention furthermore relates to a process for preparing the abovementioned PNA derivatives and to their use as pharmaceuticals and diagnostic agents.

公开(公告)号：US20040265877A1

公开(公告)日：2004-12-30

申请号：US10833490

申请日：2004-04-28

申请人： Marantech Holding, LLC

发明人： Marvin S. Antelman ,  Perry W. Antelman

IPC分类号： C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  G01N033/554 ,  G01N033/569

CPC分类号： G01N33/56911 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q1/689 ,  G01N33/5091 ,  G01N33/567 ,  G01N33/569 ,  G01N33/56966 ,  G01N33/574 ,  G01N33/57484

摘要： The invention is directed to methods for detecting, monitoring and/or diagnosing aberrant cellular proliferation, and disorders associated therewith, such as cancer, and/or infection by microorganisms. The detection, monitoring and/or diagnosis comprises contacting a compound of the invention with a cell or fluid sample. Compound binding confirms the presence of an abnormal cell or a protein associated with an abnormal cell or infection by one or more microorganisms, and/or disorders associated with infection by one or more microorganisms.

摘要翻译： 本发明涉及用于检测，监测和/或诊断异常细胞增殖以及与其相关的疾病如癌症和/或微生物感染的方法。 检测，监测和/或诊断包括将本发明的化合物与细胞或液体样品接触。 化合物结合证实存在与一种或多种微生物异常细胞或感染相关的异常细胞或蛋白质，和/或与一种或多种微生物感染相关的病症。

公开(公告)号：US20040265874A1

公开(公告)日：2004-12-30

申请号：US10828846

申请日：2004-04-20

申请人： Bio-Rad Laboratories, Inc.

发明人： Steven R. Binder ,  John Glossenger

IPC分类号： C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC分类号： G01N33/564 ,  G01N2800/24 ,  G06K9/6276 ,  G16B20/00 ,  G16B40/00 ,  G16H50/20

摘要： An array of autoantibodies is quantitated in a patient sample and analyzed toward a diagnosis of systemic autoimmune diseases. The analysis uses any of various known pattern recognition techniques, for example k-nearest neighbor analysis, to compare the array of quantitation data to sets of data previously obtained from subjects having known systemic autoimmune diseases, thereby determining the particular disease(s) that the patient is suffering from as well as the degree of confidence or likelihood of accuracy of the determination. The method is effective in identifying a single disease and also in identifying two or more diseases simultaneously present. The method is readily susceptible to automated data processing, eliminating much of the human judgment and error that were previously entailed in diagnosing these diseases.

摘要翻译： 在患者样品中量化一系列自身抗体，并对全身自身免疫性疾病的诊断进行分析。 分析使用各种已知的模式识别技术中的任何一种，例如k-最近邻分析，将定量数据阵列与先前从具有已知的系统性自身免疫性疾病的受试者获得的数据集合进行比较，从而确定特定的疾病 病人正在受苦，以及信心的程度或确定准确性的可能性。 该方法在鉴定单一疾病以及同时鉴定两种或更多种同时存在的疾病方面是有效的。 该方法容易受到自动数据处理的影响，消除了以前在诊断这些疾病方面的大部分人的判断和错误。

公开(公告)号：US20040265869A1

公开(公告)日：2004-12-30

申请号：US10812716

申请日：2004-03-30

申请人： ChondroGene Limited

发明人： Choong-Chin Liew

IPC分类号： C12Q001/68

CPC分类号： C12Q1/6809 ,  C12Q1/6883 ,  C12Q2600/158

摘要： The present invention is directed to detection and measurement of gene transcripts and their equivalent nucleic acid products in blood. Specifically provided is analysis performed on a drop of blood for detecting, diagnosing and monitoring diseases using gene-specific and/or tissue-specific primers. The present invention also describes methods by which delineation of the sequence and/or quantitation of the expression levels of disease-specific genes allows for an immediate and accurate diagnostic/prognostic test for disease or to assess the effect of a particular treatment regimen.

摘要翻译： 本发明涉及血液中基因转录物及其等价核酸产物的检测和测量。 具体提供的是使用基因特异性和/或组织特异性引物检测，诊断和监测疾病的一滴血液进行的分析。 本发明还描述了通过该方法描绘疾病特异性基因的序列和/或表达水平的定量，可以立即进行准确的疾病诊断/预后测试或评估特定治疗方案的效果。

公开(公告)号：US20040265855A1

公开(公告)日：2004-12-30

申请号：US10773100

申请日：2004-02-05

申请人： Xantos Biomedicine AG

发明人： Ulrich Pessara ,  Michael Kazinski ,  Johannes Gorl

IPC分类号： C12Q001/68

CPC分类号： C12N15/1003

摘要： The present invention relates to a method for screening a collection of nucleic acid molecules for a desired property of the nucleic acid or of a (poly)peptide encoded thereof, comprising the steps (a) automated picking of the cell collection containing the collection of nucleic acid molecules with a first robot; (b) automated lysis of the cells with a second robot; (c) automated separation of the cell DNA from the cell debris with a second robot; (d) optionally automated separation of endotoxins from the DNA with the second robot if the cells are bacteria; (e) automated transfection of the cells with the DNA obtained in step (c) or, if the cells are bacteria, with the DNA obtained in step (d) with a third robot; and (f) automated screening for the desired property with a fourth robot. Moreover, the invention relates to methods for the enhancement of the binding properties of the (poly)peptide identified by the of the screening method of the invention or encoded by the DNA identified and isolated and a method for the production of a pharmaceutical composition on the basis of (poly)peptides which can be obtained with the method of the invention and moreover the formulation of the substance obtained with a pharmaceutically acceptable carrier or dilutent.

摘要翻译： 本发明涉及一种用于筛选核酸分子集合的方法，所述核酸分子的核酸或其编码的（多）肽的所需性质包括以下步骤：（a）自动采集含有核酸集合的细胞集合 酸性分子与第一个机器人; （b）用第二机器人自动裂解细胞; （c）用第二机器人自动分离细胞DNA与细胞碎片; （d）如果细胞是细菌，则可选择性地利用第二机器人自动分离内毒素与DNA; （e）用步骤（c）中获得的DNA自动转染细胞，或者如果细胞是细菌，则用步骤（d）获得的DNA用第三机器人自动转染; 和（f）用第四机器人自动筛选所需的性能。 此外，本发明涉及增强由本发明筛选方法鉴定或由鉴定和分离的DNA编码的（多）肽的结合特性的方法，以及用于生产药物组合物的方法 （多）肽的基础，其可以用本发明的方法获得，而且用药学上可接受的载体或稀释剂制得的物质的制剂。

公开(公告)号：US20040265852A1

公开(公告)日：2004-12-30

申请号：US10734126

申请日：2003-12-15

申请人： SUGEN, INC.

发明人： Gregory Plowman ,  James Bischof

IPC分类号： C12Q001/68 ,  C07H021/04 ,  C07K014/705

CPC分类号： C12Q1/485 ,  G01N2333/9121 ,  G01N2500/02 ,  G01N2500/04 ,  G01N2500/10

摘要： The present invention relates to AUR1 and/or AUR2 polypeptides, nucleic acids encoding such polypeptides, cells, tissues and animals containing such nucleic acids, antibodies to such polypeptides, assays utilizing such polypeptides, and methods relating to all of the foregoing. Methods for treatment, diagnosis, and screening are provided for AUR1 and/or AUR2 related diseases or conditions characterized by an abnormal interaction between a AUR1 and/or AUR2 polypeptide and a AUR1 and/or AUR2 binding partner.

摘要翻译： 本发明涉及AUR1和/或AUR2多肽，编码此类多肽的核酸，含有此类核酸的细胞，组织和动物，此类多肽的抗体，利用此类多肽的测定法以及与上述所有相关的方法。 AUR1和/或AUR2相关疾病或特征为AUR1和/或AUR2多肽与AUR1和/或AUR2结合配偶体之间异常相互作用的病症的治疗，诊断和筛选方法。