发明申请
- 专利标题： Purification of protein inclusion bodies by crossflow microfiltration
- 专利标题（中）： 通过交叉流微滤法纯化蛋白质包涵体
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申请号： US10275797申请日： 2003-03-20
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公开(公告)号： US20030171560A1公开(公告)日： 2003-09-11
- 发明人: Jorg Peters
- 优先权： DE10022258.7 20000508
- 主分类号： C07K001/34
- IPC分类号： C07K001/34
摘要：
The invention relates to a process for purifying and/or concentrating inclusion bodies (refractile bodies) in a highly concentrated particle-containing solution, in which process the inclusion body-containing suspension is directed tangentially past one or more semipermeable membranes, so that the inclusion bodies are retained behind the membranes and that substances having a relatively small molecular weight may pass through the membrane and/or be adsorbed to the membrane, and a purified and/or concentrated inclusion body suspension is obtained. In the same manner, cell wall particles are removed from a inclusion body suspension. The invention further relates to the use of a crossflow microfiltration unit for purifying and/or concentrating inclusion bodies in a suspension and for removing cell wall particles from a inclusion body preparation.
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