公开(公告)号：US20020132986A1

公开(公告)日：2002-09-19

申请号：US09752138

申请日：2000-12-26

发明人： Raja R. Mamidi ,  Leticia R. Regis ,  Mauro C. Mojica ,  Hirokazu Ito ,  Takashi Goto

IPC分类号： C07K001/34 ,  C07K014/745 ,  C07K014/76

CPC分类号： A61M1/0281 ,  A61M1/3413 ,  A61M1/3687 ,  A61M2205/3324

摘要： A method is provided for removing citrate, aluminum, and other multivalent ions and contaminants from proteins by adjusting the pH of a solution containing the protein to a pH from about 7 to about 10, and diafiltering the pH-adjusted solution against aqueous solutions which have a low level of ions.

公开(公告)号：US20040167320A1

公开(公告)日：2004-08-26

申请号：US10635117

申请日：2003-08-06

发明人： Daniel E. Couto ,  Amy Laverdiere

IPC分类号： C07K001/34

CPC分类号： C07K1/34

摘要： Processes and apparati are provided for separating molecules of interest from a mixture containing them which comprises subjecting the mixture to an improved method of tangential flow filtration (TFF). The improved TFF was used to clarify, and process various feedstreams for the removal of a molecule of interest. According to a preferred embodiment, a transgenic milk feedstream is stabilized and particulate matter such as fat, casein miscelles and bacteria are removed. The method of TFF used in the current invention utilizes optimized process parameters that include temperature, transmembrane pressure, cross-flow velocity, and milk concentration. Cleaning and storage procedures were also developed to ensure long membrane life. An aseptic filtration step was also developed to remove any bacteria remaining in a clarified transgenic milk feedstream.

摘要翻译： 提供了从包含它们的混合物中分离目的分子的方法和装置，其包括使混合物经受切向流过滤（TFF）的改进方法。 改进的TFF用于澄清和处理用于去除感兴趣分子的各种进料流。 根据一个优选的实施方案，转基因奶饲料流被稳定，除去脂肪，酪蛋白，细胞和细菌等颗粒物质。 在本发明中使用的TFF的方法利用了包括温度，跨膜压力，横流速度和牛奶浓度的优化的工艺参数。 还开发了清洁和储存程序，以确保长的膜寿命。 还开发了无菌过滤步骤以除去澄清的转基因乳饲料流中剩余的任何细菌。

公开(公告)号：US20030199001A1

公开(公告)日：2003-10-23

申请号：US10419374

申请日：2003-04-21

发明人： Aldo M. Pitt ,  Sara D. Gutierrez

IPC分类号： G01N033/53 ,  C12N009/00 ,  C07K014/00 ,  C07K001/34

CPC分类号： G01N33/6851 ,  B01D61/145 ,  B01D61/18 ,  B01D63/16 ,  G01N1/405 ,  G01N33/68 ,  G01N33/6848 ,  G01N2035/1053

摘要： A system, kit and process for the separation, recovery, purification and identification of biomarkers such as peptides and the like from serum or plasma. The process is to filter the sample through a UF filtration device optionally having a vertical or substantial vertical ultrafilter membrane, especially when in a single well configuration device. The filtrate is recovered and if desired desalted using various chromatography media such as reverse phase and ion exchange media. The sample is then applied to a MALDI/TOF target or HPLC column and analyzed in a mass spectrometer the presence, absence or variation of the biomarkers. Higher recovery and concentration of these biomarkers is achieved with the present invention.

摘要翻译： 用于从血清或血浆中分离，回收，纯化和鉴定生物标志物如肽等的系统，试剂盒和方法。 该过程是通过可选地具有垂直或基本垂直超滤膜的UF过滤装置过滤样品，特别是在单一孔配置装置中。 回收滤液，如果需要，使用各种色谱介质如反相和离子交换介质脱盐。 然后将样品应用于MALDI / TOF靶或HPLC柱，并在质谱仪中分析生物标志物的存在，不存在或变异。 通过本发明可以实现这些生物标志物的更高的恢复和浓度。

公开(公告)号：US20030171560A1

公开(公告)日：2003-09-11

申请号：US10275797

申请日：2003-03-20

发明人： Jorg Peters

IPC分类号： C07K001/34

CPC分类号： C07K1/34

摘要： The invention relates to a process for purifying and/or concentrating inclusion bodies (refractile bodies) in a highly concentrated particle-containing solution, in which process the inclusion body-containing suspension is directed tangentially past one or more semipermeable membranes, so that the inclusion bodies are retained behind the membranes and that substances having a relatively small molecular weight may pass through the membrane and/or be adsorbed to the membrane, and a purified and/or concentrated inclusion body suspension is obtained. In the same manner, cell wall particles are removed from a inclusion body suspension. The invention further relates to the use of a crossflow microfiltration unit for purifying and/or concentrating inclusion bodies in a suspension and for removing cell wall particles from a inclusion body preparation.

摘要翻译： 本发明涉及一种用于在高浓度含有颗粒的溶液中净化和/或浓缩包涵体（折射体）的方法，其中含有夹杂物的悬浮液切向地穿过一个或多个半透膜，使得包含 身体保留在膜的后面，并且具有相对较小分子量的物质可以通过膜和/或吸附到膜上，并且获得纯化和/或浓缩的包涵体悬浮液。 以相同的方式，从包涵体悬浮液中除去细胞壁颗粒。 本发明还涉及使用交叉流微量微滤单元来净化和/或浓缩悬浮液中的包涵体和从包涵体制剂中除去细胞壁颗粒。

公开(公告)号：US20020009781A1

公开(公告)日：2002-01-24

申请号：US09760397

申请日：2001-01-12

发明人： Marc D. Better ,  Patrick D. Gavit

IPC分类号： C12P001/04 ,  C12P021/02 ,  C07K001/14 ,  C07K001/34 ,  C12P021/04 ,  C07K001/00 ,  A61K038/00

CPC分类号： C12N15/62 ,  C07K14/4742 ,  C07K14/51 ,  C07K2319/00 ,  C07K2319/35 ,  C12N15/70

摘要： The present invention provides improved methods for the production of recombinant peptides from bacterial cells.

公开(公告)号：US20040116679A1

公开(公告)日：2004-06-17

申请号：US10474065

申请日：2003-10-03

发明人： Preben Busch Knullnigsfeldt ,  Chris Burt ,  Mark Allan Litchfield

IPC分类号： A23J001/00 ,  A23C017/00 ,  A23C021/00 ,  C07K001/34

CPC分类号： A23J1/205 ,  A23C9/1425 ,  A23C21/00

摘要： A method and a plant for the separation of fat from proteins in a whey material to obtain a whey protein isolate (WPI) with a low fat content comprising the combination of a centrifugal separator and microfiltration (MF). The material fed to the separator contains the MF-retentate not passing through the microfilter. The combination improves the efficiency of the centrifugal separator and increases the yield of valuable WPI.

摘要翻译： 一种用于从乳清材料中的蛋白质分离脂肪的方法和设备，以获得包含离心分离器和微滤（MF）的组合的低脂肪含量的乳清蛋白分离物（WPI）。 进料到分离器的材料含有不通过微过滤器的MF滞留物。 该组合提高了离心分离器的效率，并提高了有价值WPI的产率。

公开(公告)号：US20030191292A1

公开(公告)日：2003-10-09

申请号：US10304902

申请日：2002-11-26

申请人： Octapharma AG, a Corporation of Switzerland

发明人： Stefan Winge

IPC分类号： C07K001/34 ,  C08B037/00 ,  C07K016/00 ,  C07K014/76 ,  C07K014/745

CPC分类号： C12N9/644 ,  C07K1/34 ,  C07K14/4717 ,  C07K14/755 ,  C07K14/76 ,  C07K14/8128 ,  C12Y304/21022

摘要： Filtration methods comprise virus-filtering a solution containing a least one macromolecule. The total salt content of the solution for virus-filtering is within the range of from about 0.2 M up to saturation with the salt. Salts that can be used in the filtering methods include sodium chloride, potassium chloride, sodium acetate, sodium citrate, sodium phosphate, potassium dyhydrophosphate and combinations thereof.

摘要翻译： 过滤方法包括病毒过滤含有至少一种大分子的溶液。 用于病毒过滤的溶液的总盐含量在约0.2M至饱和盐的范围内。 可用于过滤方法的盐包括氯化钠，氯化钾，乙酸钠，柠檬酸钠，磷酸钠，二氢磷酸氢钾及其组合。