发明申请
- 专利标题: Method for recombinant production of polypeptides
- 专利标题(中): 重组生产多肽的方法
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申请号: US10866567申请日: 2004-06-12
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公开(公告)号: US20050003485A1公开(公告)日: 2005-01-06
- 发明人: Rainer Gruenbeck , Erhard Kopetzki , Friedrich Popp
- 申请人: Rainer Gruenbeck , Erhard Kopetzki , Friedrich Popp
- 优先权: EP03012295.6 20030612
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C12N1/21 ; C12P21/00 ; C12P21/02 ; C07K14/00
摘要:
A method for the recombinant production of a polypeptide by expressing a nucleic acid encoding said polypeptide in a microbial host cell, forming in the cytoplasm of said host cell inclusion bodies containing said polypeptide, and isolating, solubilizing and naturing said polypeptide, characterized in that after fermentation the host cell or the host cell content is incubated at a temperature of 40° C. or higher for at least 10 minutes and subsequently insoluble polypeptide is isolated from the host cell, this method providing an improved yield in inclusion bodies containing the desired polypeptide.
公开/授权文献
- US07034119B2 Method for recombinant production of polypeptides 公开/授权日:2006-04-25
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