发明申请
US20050124035A1 Method for producing alpha-L-aspartyl-L-phenylalanine-beta-ester and methd for producing alpha-L-aspartyl-l-phenylalanine-alpha-methyl esther
有权
生产α-L-天冬氨酰基-L-苯丙氨酸-β-酯的方法和用于生产α-L-天冬氨酰-1-苯丙氨酸-α-甲基雌酮的方法
- 专利标题: Method for producing alpha-L-aspartyl-L-phenylalanine-beta-ester and methd for producing alpha-L-aspartyl-l-phenylalanine-alpha-methyl esther
- 专利标题(中): 生产α-L-天冬氨酰基-L-苯丙氨酸-β-酯的方法和用于生产α-L-天冬氨酰-1-苯丙氨酸-α-甲基雌酮的方法
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申请号: US10876673申请日: 2004-06-28
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公开(公告)号: US20050124035A1公开(公告)日: 2005-06-09
- 发明人: Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
- 申请人: Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
- 申请人地址: JP Tokyo 104-8315
- 专利权人: Ajinomoto Co., Inc.
- 当前专利权人: Ajinomoto Co., Inc.
- 当前专利权人地址: JP Tokyo 104-8315
- 优先权: JP2003-016764 20030124; JP2003-201819 20030725
- 主分类号: A23L27/30
- IPC分类号: A23L27/30 ; C07K5/072 ; C12N15/57 ; C12P21/02 ; C12P21/06 ; C07K5/04 ; C12N1/21
摘要:
A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.