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1.发明授权Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权制备α-L-天冬氨酰基-L-苯丙氨酸的方法和制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的方法公开(公告)号：US07745172B2
公开(公告)日：2010-06-29
申请号：US11970203
申请日：2008-01-07
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/06
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.
摘要翻译： 制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯（也称为α-L- - （取代的天冬氨酰基）-L-苯丙氨酸）的方法，其为α-L 提供了天竺葵酰-L-苯丙氨酸-α-甲酯（也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;产品名称：阿斯巴甜），容易地以高产率和低成本而不经过复合合成方法。 此外，提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易且廉价且高产率的制备方法。 使用具有催化反应能力的酶或酶的物质由L-天冬氨酸-α，β-二酯和L-苯丙氨酸制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯 其中L-苯丙氨酸对L-天冬氨酸-α，bgr-siester的“酯”位点不发生亲核攻击，但对其α-酯位点进行亲核攻击。
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2.发明授权Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权生产α-L-天冬氨酰-L-苯丙氨酸-β-酯的方法及其制备α-天冬氨酰-L-苯丙氨酸-α-甲酯的方法公开(公告)号：US07361458B2
公开(公告)日：2008-04-22
申请号：US10876673
申请日：2004-06-28
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.
摘要翻译： 制备α-L-天冬氨酰基-L-苯丙氨酸-β-酯（也称为α-L-（β-邻位取代的天冬氨酰基）-L-苯丙氨酸）的方法，其为α-L-天冬氨酰基 提供了L-苯丙氨酸-α-甲酯（也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;商品名：阿斯巴甜），容易地以高产率和低成本地经历复合合成方法。 此外，提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易，便宜且高产量的制备方法。 使用具有催化反应能力的酶或含酶物质由L-天冬氨酸-α，β-二酯和L-苯丙氨酸生产α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯，其中 L-苯丙氨酸不对L-天冬氨酸-α，β-二酯的β-酯位点进行亲核攻击，但对其α-酯位点进行亲核攻击。
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3.发明授权Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权制备α-L-天冬氨酰基-L-苯丙氨酸的方法和制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的方法公开(公告)号：US08361748B2
公开(公告)日：2013-01-29
申请号：US13528162
申请日：2012-06-20
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/06
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.
摘要翻译： 通过从L-天冬氨酸-α，二酯和L形成α-L-天冬氨酰基-L-苯丙氨酸 - 乙酸酯制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯的方法 - 苯丙氨酸，其使用具有通过肽键将L-苯丙氨酸选择性地连接到L-天冬氨酸-α，α-二酯的α-酯位点的酶或酶的物质。
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4.发明授权Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权制备α-L-天冬氨酰基-L-苯丙氨酸的方法和制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的方法公开(公告)号：US08034584B2
公开(公告)日：2011-10-11
申请号：US12749966
申请日：2010-03-30
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/06
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-αβ-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-αβ-diester through a peptide bond.
摘要翻译： 通过从L-天冬氨酸-α和bgr-二酯和L-苯丙氨酸形成α-L-天冬氨酰基-L-苯丙氨酸 - 乙酸酯制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯的方法 使用具有通过肽键将L-苯丙氨酸选择性地连接到L-天冬氨酸-α和bgr-二酯的α-酯位点的酶或酶的物质。
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公开(公告)号：US08389240B2
公开(公告)日：2013-03-05
申请号：US13528126
申请日：2012-06-20
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/06
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.
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6.发明授权Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权制备α-L-天冬氨酰基-L-苯丙氨酸的方法和制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的方法公开(公告)号：US08247193B2
公开(公告)日：2012-08-21
申请号：US13198075
申请日：2011-08-04
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/06
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.
摘要翻译： 通过从L-天冬氨酸-α，二酯和L形成α-L-天冬氨酰基-L-苯丙氨酸 - 乙酸酯制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯的方法 - 苯丙氨酸，其使用具有通过肽键将L-苯丙氨酸选择性地连接到L-天冬氨酸-α，α-二酯的α-酯位点的酶或酶的物质。
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7.发明申请Method for producing alpha-L-aspartyl-L-phenylalanine-beta-ester and methd for producing alpha-L-aspartyl-l-phenylalanine-alpha-methyl esther 有权生产α-L-天冬氨酰基-L-苯丙氨酸-β-酯的方法和用于生产α-L-天冬氨酰-1-苯丙氨酸-α-甲基雌酮的方法公开(公告)号：US20050124035A1
公开(公告)日：2005-06-09
申请号：US10876673
申请日：2004-06-28
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.
摘要翻译： 制备α-L-天冬氨酰基-L-苯丙氨酸-β-酯（也称为α-L-（β-邻位取代的天冬氨酰基）-L-苯丙氨酸）的方法，其为α-L-天冬氨酰基 提供了L-苯丙氨酸-α-甲酯（也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;商品名：阿斯巴甜），容易地以高产率和低成本地经历复合合成方法。 此外，提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易，便宜且高产量的制备方法。 使用具有催化反应能力的酶或含酶物质由L-天冬氨酸-α，β-二酯和L-苯丙氨酸生产α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯，其中 L-苯丙氨酸不对L-天冬氨酸-α，β-二酯的β-酯位点进行亲核攻击，但对其α-酯位点进行亲核攻击。
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8.发明授权公开(公告)号：US08389241B2
公开(公告)日：2013-03-05
申请号：US13611827
申请日：2012-09-12
申请人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
发明人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。
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9.发明授权公开(公告)号：US08329428B2
公开(公告)日：2012-12-11
申请号：US13211834
申请日：2011-08-17
申请人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
发明人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。
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公开(公告)号：US08084227B2
公开(公告)日：2011-12-27
申请号：US11330076
申请日：2006-01-12
申请人： Kenzo Yokozeki , Isao Abe , Seiichi Hara
发明人： Kenzo Yokozeki , Isao Abe , Seiichi Hara
CPC分类号： C12P21/02
摘要： The present invention provides a method for producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide from an amino acid ester and an amino acid, microbial cells separated from the culture, or treated microbial cell product.
摘要翻译： 本发明提供了一种通过工业上有利且简单的途径以低成本获得的起始材料制备二肽的方法。 通过使用具有从氨基酸酯和氨基酸产生二肽的能力的微生物培养物，从培养物分离的微生物细胞或经处理的微生物细胞产物，由氨基酸酯和氨基酸产生二肽。
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