发明申请
US20050136466A1 Sensor using impedance change to detect the end-point for PCR DNA amplification
失效
传感器使用阻抗变化来检测PCR DNA扩增的终点
- 专利标题: Sensor using impedance change to detect the end-point for PCR DNA amplification
- 专利标题(中): 传感器使用阻抗变化来检测PCR DNA扩增的终点
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申请号: US11020731申请日: 2004-12-21
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公开(公告)号: US20050136466A1公开(公告)日: 2005-06-23
- 发明人: Robin Miles , Phillip Belgrader , Christopher Fuller
- 申请人: Robin Miles , Phillip Belgrader , Christopher Fuller
- 专利权人: The Regents of the University of California
- 当前专利权人: The Regents of the University of California
- 主分类号: C12M1/34
- IPC分类号: C12M1/34 ; C12P19/34 ; C12Q1/68 ; G01N27/02
摘要:
Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.