发明申请
- 专利标题: E2F oligonucleotide decoy molecules
- 专利标题(中): E2F寡核苷酸诱饵分子
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申请号: US10960165申请日: 2004-10-06
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公开(公告)号: US20050164240A1公开(公告)日: 2005-07-28
- 发明人: Christi Parham , Leslie McEvoy , Jie Zhang , Xiaoping Rao
- 申请人: Christi Parham , Leslie McEvoy , Jie Zhang , Xiaoping Rao
- 申请人地址: US CA South San Francisco
- 专利权人: Corgentech, Inc.
- 当前专利权人: Corgentech, Inc.
- 当前专利权人地址: US CA South San Francisco
- 主分类号: A61K38/00
- IPC分类号: A61K38/00 ; C12N15/113 ; C12Q1/68
摘要:
The invention concerns E2F oligonucleotide decoy molecules with improved properties. In particular, the invention concerns a double-stranded E2F decoy oligodeoxynucleotide (dsODN) molecule comprising a core sequence that is capable of specific binding to an E2F transcription factor, flanked by 5′ and 3′ sequences, wherein (i) the core sequence consists of about 5 to 12 base pairs; (ii) the molecule comprises an about 12 to 28 base-pair long double-stranded region composed of two fully complementary strands; and (iii) the E2F dsODN binds to said E2F transcription factor with a binding affinity that is at least about 5-fold of the binding affinity of a reference decoy molecule shown in FIG. 1 (SEQ ID NOS: 1 and 2), as determined by a competitive gel mobility shift binding assay performed on nuclear extract from THP-1 cells.
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