公开(公告)号：US20050164240A1

公开(公告)日：2005-07-28

申请号：US10960165

申请日：2004-10-06

申请人： Christi Parham ,  Leslie McEvoy ,  Jie Zhang ,  Xiaoping Rao

发明人： Christi Parham ,  Leslie McEvoy ,  Jie Zhang ,  Xiaoping Rao

IPC分类号： A61K38/00 ,  C12N15/113 ,  C12Q1/68

CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/111 ,  C12N2310/13

摘要： The invention concerns E2F oligonucleotide decoy molecules with improved properties. In particular, the invention concerns a double-stranded E2F decoy oligodeoxynucleotide (dsODN) molecule comprising a core sequence that is capable of specific binding to an E2F transcription factor, flanked by 5′ and 3′ sequences, wherein (i) the core sequence consists of about 5 to 12 base pairs; (ii) the molecule comprises an about 12 to 28 base-pair long double-stranded region composed of two fully complementary strands; and (iii) the E2F dsODN binds to said E2F transcription factor with a binding affinity that is at least about 5-fold of the binding affinity of a reference decoy molecule shown in FIG. 1 (SEQ ID NOS: 1 and 2), as determined by a competitive gel mobility shift binding assay performed on nuclear extract from THP-1 cells.

摘要翻译： 本发明涉及具有改进性质的E2F寡核苷酸诱饵分子。 特别地，本发明涉及双链E2F诱饵寡脱氧核苷酸（dsODN）分子，其包含能够与侧翼为5'和3'序列的E2F转录因子特异性结合的核心序列，其中（i）核心序列组成 约5至12个碱基对; （ii）分子包含由两个完全互补的链组成的约12至28个碱基对长双链区域; 和（iii）E2F dsODN以与图1所示的参考诱饵分子的结合亲和力的至少约5倍的结合亲和力结合所述E2F转录因子。 1（SEQ ID NO：1和2），其通过对来自THP-1细胞的核提取物进行的竞争性凝胶迁移率转移结合测定法测定。