发明申请
- 专利标题: Methods for the recombinant production of antifusogenic peptides
- 专利标题(中): 重组生产抗融合肽的方法
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申请号: US10716095申请日: 2003-11-18
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公开(公告)号: US20050191729A1公开(公告)日: 2005-09-01
- 发明人: Alexandra Kaczmarek , Erhard Kopetzki , Christian Schantz , Stefan Seeber
- 申请人: Alexandra Kaczmarek , Erhard Kopetzki , Christian Schantz , Stefan Seeber
- 优先权: EP03000988.0 20030117; EP02025618.6 20021119
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; A61K38/00 ; C07K14/16 ; C12P21/00 ; C12P21/02 ; C12Q1/70 ; C07H21/04 ; C07K14/47
摘要:
A process for the recombinant production of an antifusogenic peptide by expression of a nucleic acid encoding the antifusogenic peptide as a repeat peptide in a microbial host cell to form inclusion bodies which comprise said repeat peptide, comprising the steps of washing the inclusion bodies with a denaturing agent at a pH value of at or below pH 6.5, solubilizing the washed inclusion bodies at a pH value of at least pH 9, and cleaving said repeat peptide to obtain said antifusogenic peptide.
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