发明申请
- 专利标题： Method for isolating ligands
- 专利标题（中）： 分离配体的方法
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申请号： US10505929申请日： 2003-02-27
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公开(公告)号： US20050221381A1公开(公告)日： 2005-10-06
- 发明人: Christof Klade , Juliane Schalich , Oresta Vytvytska , Wolfgang Zauner , Max Birnstiel , Gerald Aichinger , Alexander Otava , Frank Mattner
- 申请人： Christof Klade , Juliane Schalich , Oresta Vytvytska , Wolfgang Zauner , Max Birnstiel , Gerald Aichinger , Alexander Otava , Frank Mattner
- 优先权： AT316/2002 20020228; AT1376/2002 20020911
- 国际申请： PCT/EP03/02005 WO 20030227
- 主分类号： C12Q1/02
- IPC分类号： C12Q1/02 ; A61K35/12 ; A61K38/00 ; A61K39/00 ; A61K39/245 ; A61P31/12 ; A61P43/00 ; C07K7/00 ; C07K7/06 ; C07K7/08 ; C07K14/00 ; C07K14/045 ; C07K16/28 ; C07K14/74 ; G01N33/53
摘要：
Described is a method for isolating ligands which have a binding capacity to a MHC/HLA molecule or a complex comprising said ligand and said MHC/HLA molecule which method comprises the following steps:—providing a pool of ligands, said pool containing ligands which bind to said MHC/HLA molecule and ligands which do not bind to said MHC/HLA molecule,—contacting said MHC/HLA molecule with said pool of ligands whereby a ligand which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said ligand and said MHC/HLA molecule is formed,—detecting and optionally separating said complex from the ligands which do not bind to said MHC/HLA molecule and—optionally isolating and characterising the ligand from said complex as well as a method for isolating T cell epitopes which have a binding capacity to a MHC/HLA molecule.