A method of fabricating a donor substrate and a method of fabricating an organic light emitting display (OLED) using the donor substrate. The method of fabricating the donor substrate includes preparing a base substrate that includes at least one transfer region and at least one non-transfer region, forming a light-to-heat conversion layer on the base substrate and depositing a transfer layer selectively on the light-to-heat conversion layer and in the at least one transfer region of the base substrate using a shadow mask. To then make the OLED, laser induced thermal imaging is used to transfer the patterned transfer layer from the donor substrate to display regions in an acceptor substrate.