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发明申请
US20060094008A1 Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers 有权
用于检测细胞角蛋白的核酸扩增引物和使用引物的检查方法

Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers
摘要：
It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.
摘要（中）：

旨在提供用于检测人细胞角蛋白（Human CK）的基因扩增反应中使用的新引物。 一种含有寡核苷酸的引物，其包含选自SEQ ID NO：1所示的碱基序列的270〜1375位的碱基序列及其互补链的区域， 构建SEQ ID NOS：2至341。 此外，含有包含选自SEQ ID NO：342所示的碱基序列的270〜930区域的碱基序列的寡核苷酸的引物及其互补链的区域，例如任何 构建为SEQ ID NO：343〜434。 此外，含有选自SEQ ID NO：435表示的碱基序列的340〜490个区域或495〜1050个区域的碱基序列的寡核苷酸的引物及其互补链的区域 并且由例如SEQ ID NO：436至474中的任一个表示。

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