Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers
    1.
    发明申请
    Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers 有权
    用于检测细胞角蛋白的核酸扩增引物和使用引物的检查方法

    公开(公告)号:US20060094008A1

    公开(公告)日:2006-05-04

    申请号:US10514776

    申请日:2003-05-20

    IPC分类号: C12Q1/68 C07H21/04 C12P19/34

    摘要: It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.

    摘要翻译: 旨在提供用于检测人细胞角蛋白(Human CK)的基因扩增反应中使用的新引物。 一种含有寡核苷酸的引物,其包含选自SEQ ID NO:1所示的碱基序列的270〜1375位的碱基序列及其互补链的区域, 构建SEQ ID NOS:2至341。 此外,含有包含选自SEQ ID NO:342所示的碱基序列的270〜930区域的碱基序列的寡核苷酸的引物及其互补链的区域,例如任何 构建为SEQ ID NO:343〜434。 此外,含有选自SEQ ID NO:435表示的碱基序列的340〜490个区域或495〜1050个区域的碱基序列的寡核苷酸的引物及其互补链的区域 并且由例如SEQ ID NO:436至474中的任一个表示。

    Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers
    2.
    发明授权
    Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers 有权
    用于检测细胞角蛋白的核酸扩增引物和使用引物的检查方法

    公开(公告)号:US08076080B2

    公开(公告)日:2011-12-13

    申请号:US10514776

    申请日:2003-05-20

    IPC分类号: C12Q1/68 C12P19/34 C07H21/04

    摘要: It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.

    摘要翻译: 旨在提供用于检测人细胞角蛋白(Human CK)的基因扩增反应中使用的新引物。 一种含有寡核苷酸的引物,其包含选自SEQ ID NO:1所示的碱基序列的270〜1375位的碱基序列及其互补链的区域, 构建SEQ ID NOS:2至341。 此外,含有包含选自SEQ ID NO:342所示的碱基序列的270〜930区域的碱基序列的寡核苷酸的引物及其互补链的区域,例如任何 构建为SEQ ID NO:343〜434。 此外,含有选自SEQ ID NO:435表示的碱基序列的340〜490个区域或495〜1050个区域的碱基序列的寡核苷酸的引物及其互补链的区域 并且由例如SEQ ID NO:436至474中的任一个表示。

    METHOD AND KIT FOR DETERMINING THE AMOUNT OF DNA BINDING PROTEIN
    4.
    发明申请
    METHOD AND KIT FOR DETERMINING THE AMOUNT OF DNA BINDING PROTEIN 审中-公开
    用于测定DNA结合蛋白质量的方法和试剂盒

    公开(公告)号:US20090004749A1

    公开(公告)日:2009-01-01

    申请号:US12163431

    申请日:2008-06-27

    IPC分类号: G01N33/00

    CPC分类号: G01N33/5308 G01N33/6872

    摘要: A kit for determining a quantity of a target DNA binding protein in a liquid sample by using a fluorescence correlation spectroscopy, comprising a first measuring reagent including a fluorescent-labeled nucleic acid probe and a first unlabeled nucleic acid probe, a second measuring reagent including the fluorescent-labeled nucleic acid probe and a second unlabeled nucleic acid probe, wherein the target DNA binding protein is capable of binding to the fluorescent-labeled nucleic acid probe and the first unlabeled nucleic acid probe, and is not capable of binding to the second unlabeled nucleic acid probe; and a method for determining a quantity of a target DNA binding protein in a liquid sample is also disclosed.

    摘要翻译: 一种用于通过使用荧光相关光谱测定液体样品中的靶DNA结合蛋白的量的试剂盒,其包括含有荧光标记的核酸探针和第一未标记的核酸探针的第一测定试剂,包含第 荧光标记的核酸探针和第二个未标记的核酸探针,其中靶DNA结合蛋白能够结合荧光标记的核酸探针和第一个未标记的核酸探针,并且不能结合第二个未标记的核酸探针 核酸探针; 还公开了一种用于测定液体样品中目标DNA结合蛋白的量的方法。

    Method for detecting a target substance by using a nucleic acid probe
    9.
    发明申请
    Method for detecting a target substance by using a nucleic acid probe 审中-公开
    通过使用核酸探针检测目标物质的方法

    公开(公告)号:US20060234253A1

    公开(公告)日:2006-10-19

    申请号:US11244045

    申请日:2005-10-06

    IPC分类号: C12Q1/68 C07H21/04

    摘要: A method for detecting a target substance is described, which comprises the steps of: forming a first complex comprising (a) a first nucleic acid probe immobilized on a first solid phase and having a single-strand portion, (b) a nucleic acid ligand which has a binding section as a base sequence hybridizable with the single-strand portion of the first nucleic acid probe and which has a target-binding substance capable of binding to the target substance, (c) the target substance, and (d) a labeling substance capable of binding to the target substance; separating, from the first complex, a second complex comprising the nucleic acid ligand, the target substance and the labeling substance; and detecting the target substance on the basis of the labeling substance in the second complex.

    摘要翻译: 描述了用于检测目标物质的方法,其包括以下步骤:形成第一配合物,其包含(a)固定在第一固相上并具有单链部分的第一核酸探针,(b)核酸配体 其具有与第一核酸探针的单链部分杂交的碱基序列的结合部分,其具有能够结合目标物质的靶结合物质,(c)靶物质,和(d) 能够结合目标物质的标记物质; 从第一复合物中分离出包含核酸配体,靶物质和标记物质的第二复合物; 并基于第二配合物中的标记物质检测目标物质。

    Data processing apparatus, data processing method, program, and ink jet printing system
    10.
    发明授权
    Data processing apparatus, data processing method, program, and ink jet printing system 有权
    数据处理装置,数据处理方法,程序和喷墨打印系统

    公开(公告)号:US07114795B2

    公开(公告)日:2006-10-03

    申请号:US10647204

    申请日:2003-08-26

    IPC分类号: B41J2/165

    CPC分类号: B41J11/0065 G06K15/181

    摘要: An ink jet printing system is provided which has an ink jet printing apparatus and a host and which alleviates a data processing load on the ink jet printing apparatus, prevents a reduction in a printing operation speed, and realizes a cost reduction. The ink jet printing apparatus of this invention has an ink receiving member for receiving waste ink ejected onto an overrunning area outside edges of a print medium and an accumulated value memory section for storing an accumulated value equivalent to waste ink volumes ejected onto the ink receiving member. The host includes a waste ink volume setting section for obtaining a value equivalent to a waste ink volume and a data sending section for sending data to the ink jet printing apparatus. The accumulated value memory section of the ink jet printing apparatus stores the accumulated value equivalent to waste ink volumes obtained by the host.

    摘要翻译: 提供一种喷墨打印系统,其具有喷墨打印设备和主机,并且其减轻了喷墨打印设备上的数据处理负担,防止了打印操作速度的降低,并且实现了成本降低。 本发明的喷墨打印设备具有用于接收喷射到打印介质外边缘的超越区域上的废墨的墨接收部件和用于存储与喷墨到墨接收部件上的废墨量相当的累积值的累积值存储部 。 主机包括用于获得与废墨量相当的值的废墨量设置部分和用于向喷墨打印设备发送数据的数据发送部分。 喷墨打印装置的累积值存储部存储与主机获得的废墨量相当的累积值。