摘要:
It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.
摘要翻译:旨在提供用于检测人细胞角蛋白(Human CK)的基因扩增反应中使用的新引物。 一种含有寡核苷酸的引物,其包含选自SEQ ID NO:1所示的碱基序列的270〜1375位的碱基序列及其互补链的区域, 构建SEQ ID NOS:2至341。 此外,含有包含选自SEQ ID NO:342所示的碱基序列的270〜930区域的碱基序列的寡核苷酸的引物及其互补链的区域,例如任何 构建为SEQ ID NO:343〜434。 此外,含有选自SEQ ID NO:435表示的碱基序列的340〜490个区域或495〜1050个区域的碱基序列的寡核苷酸的引物及其互补链的区域 并且由例如SEQ ID NO:436至474中的任一个表示。
摘要:
It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.
摘要翻译:旨在提供用于检测人细胞角蛋白(Human CK)的基因扩增反应中使用的新引物。 一种含有寡核苷酸的引物,其包含选自SEQ ID NO:1所示的碱基序列的270〜1375位的碱基序列及其互补链的区域, 构建SEQ ID NOS:2至341。 此外,含有包含选自SEQ ID NO:342所示的碱基序列的270〜930区域的碱基序列的寡核苷酸的引物及其互补链的区域,例如任何 构建为SEQ ID NO:343〜434。 此外,含有选自SEQ ID NO:435表示的碱基序列的340〜490个区域或495〜1050个区域的碱基序列的寡核苷酸的引物及其互补链的区域 并且由例如SEQ ID NO:436至474中的任一个表示。
摘要:
An aircraft ice protection system is provided for preventing ice accretion on a wing of an aircraft or removing the accreted ice. Bleed air extracted from a main engine of the aircraft and air introduced from an air intake installed on an airframe and heated by a heat source of the airframe of the aircraft are selectively supplied to a hot air chamber formed inside the wing, thereby carrying out ice protection.
摘要:
A kit for determining a quantity of a target DNA binding protein in a liquid sample by using a fluorescence correlation spectroscopy, comprising a first measuring reagent including a fluorescent-labeled nucleic acid probe and a first unlabeled nucleic acid probe, a second measuring reagent including the fluorescent-labeled nucleic acid probe and a second unlabeled nucleic acid probe, wherein the target DNA binding protein is capable of binding to the fluorescent-labeled nucleic acid probe and the first unlabeled nucleic acid probe, and is not capable of binding to the second unlabeled nucleic acid probe; and a method for determining a quantity of a target DNA binding protein in a liquid sample is also disclosed.
摘要:
A treatment solution for preparing a sample solution for nucleic acid amplification reaction is described. The treatment solution comprises dimethyl sulfoxide and aqueous solvent. The sample solution prepared by treating a biological sample with the treatment solution is used in the amplification reaction of a nucleic acid, whereby the nucleic acid can be efficiently recovered from a biological sample, and the influence of an inhibitor during nucleic acid amplification can be effectively reduced.
摘要:
A treatment solution for preparing a sample solution for nucleic acid amplification reaction is described. The treatment solution comprises dimethyl sulfoxide and aqueous solvent. The sample solution prepared by treating a biological sample with the treatment solution is used in the amplification reaction of a nucleic acid, whereby the nucleic acid can be efficiently recovered from a biological sample, and the influence of an inhibitor during nucleic acid amplification can be effectively reduced.
摘要:
A printing apparatus of the invention acquires a velocity and an acceleration of a printing medium when a rear end of the printing medium passes through a detection lever. A correction amount for the rear end of the printing medium is determined in accordance with the velocity and the acceleration of the printing medium. The correction amount is a transportation amount of the printing medium during a physical return time after the rear end of the printing medium passes through the detection lever until the detection lever is detected by a sensor. A remaining region from a printing position of a print head up to the rear end of the printing medium is controlled in accordance with the correction amount for the rear end of printing medium.
摘要:
An aircraft ice protection system is provided for preventing ice accretion on a wing of an aircraft or removing the accreted ice. Bleed air extracted from a main engine of the aircraft and air introduced from an air intake installed on an airframe and heated by a heat source of the airframe of the aircraft are selectively supplied to a hot air chamber formed inside the wing, thereby carrying out ice protection.
摘要:
A method for detecting a target substance is described, which comprises the steps of: forming a first complex comprising (a) a first nucleic acid probe immobilized on a first solid phase and having a single-strand portion, (b) a nucleic acid ligand which has a binding section as a base sequence hybridizable with the single-strand portion of the first nucleic acid probe and which has a target-binding substance capable of binding to the target substance, (c) the target substance, and (d) a labeling substance capable of binding to the target substance; separating, from the first complex, a second complex comprising the nucleic acid ligand, the target substance and the labeling substance; and detecting the target substance on the basis of the labeling substance in the second complex.
摘要:
An ink jet printing system is provided which has an ink jet printing apparatus and a host and which alleviates a data processing load on the ink jet printing apparatus, prevents a reduction in a printing operation speed, and realizes a cost reduction. The ink jet printing apparatus of this invention has an ink receiving member for receiving waste ink ejected onto an overrunning area outside edges of a print medium and an accumulated value memory section for storing an accumulated value equivalent to waste ink volumes ejected onto the ink receiving member. The host includes a waste ink volume setting section for obtaining a value equivalent to a waste ink volume and a data sending section for sending data to the ink jet printing apparatus. The accumulated value memory section of the ink jet printing apparatus stores the accumulated value equivalent to waste ink volumes obtained by the host.