发明申请
- 专利标题： Bisulfite conversion of DNA
- 专利标题（中）： 亚硫酸氢盐转化DNA
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申请号： US10964167申请日： 2004-10-12
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公开(公告)号： US20060134643A1公开(公告)日： 2006-06-22
- 发明人: Kurt Berlin , Matthias Ballhause , Karen Cardon
- 申请人： Kurt Berlin , Matthias Ballhause , Karen Cardon
- 优先权： DE10029915.6 20010619; DE10347396.3 20031009; DE10347397.1 20031009; DE10347400.5 20031009; DE10347399.8 20031009
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C07H21/04
摘要：
The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods.
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