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公开(公告)号：US20110250601A1
公开(公告)日：2011-10-13
申请号：US13159323
申请日：2011-06-13
申请人： Kurt Berlin , Matthias Ballhause , Karen Cardon
发明人： Kurt Berlin , Matthias Ballhause , Karen Cardon
CPC分类号： C12Q1/6827 , C12Q2533/101 , Y10T436/143333 , C12Q2527/125 , C12Q2523/125 , C12Q2531/125
摘要： The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods.
摘要翻译： 本发明提供了DNA的亚硫酸氢盐转化的改进方法，并且有助于分析基因组DNA的胞嘧啶甲基化。 相对于现有技术的方法，变性溶剂，新反应条件和新的纯化方法的新型组合为DNA的亚硫酸氢盐转化提供了惊人的有效方法。 随后可以通过许多不同的方法分析转化的DNA。
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公开(公告)号：US07968295B2
公开(公告)日：2011-06-28
申请号：US12410389
申请日：2009-03-24
申请人： Kurt Berlin , Matthias Ballhause , Karen Cardon
发明人： Kurt Berlin , Matthias Ballhause , Karen Cardon
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2533/101 , Y10T436/143333 , C12Q2527/125 , C12Q2523/125 , C12Q2531/125
摘要： The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods.
摘要翻译： 本发明提供了DNA的亚硫酸氢盐转化的改进方法，并且有助于分析基因组DNA的胞嘧啶甲基化。 相对于现有技术的方法，变性溶剂，新反应条件和新的纯化方法的新型组合为DNA的亚硫酸氢盐转化提供了惊人的有效方法。 随后可以通过许多不同的方法分析转化的DNA。
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公开(公告)号：US20060134643A1
公开(公告)日：2006-06-22
申请号：US10964167
申请日：2004-10-12
申请人： Kurt Berlin , Matthias Ballhause , Karen Cardon
发明人： Kurt Berlin , Matthias Ballhause , Karen Cardon
CPC分类号： C12Q1/6827 , C12Q2533/101 , Y10T436/143333 , C12Q2527/125 , C12Q2523/125 , C12Q2531/125
摘要： The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods.
摘要翻译： 本发明提供了DNA的亚硫酸氢盐转化的改进方法，并且有助于分析基因组DNA的胞嘧啶甲基化。 相对于现有技术的方法，变性溶剂，新反应条件和新的纯化方法的新型组合为DNA的亚硫酸氢盐转化提供了惊人的有效方法。 随后可以通过许多不同的方法分析转化的DNA。
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公开(公告)号：US08257950B2
公开(公告)日：2012-09-04
申请号：US13159323
申请日：2011-06-13
申请人： Kurt Berlin , Matthias Ballhause , Karen Cardon
发明人： Kurt Berlin , Matthias Ballhause , Karen Cardon
CPC分类号： C12Q1/6827 , C12Q2533/101 , Y10T436/143333 , C12Q2527/125 , C12Q2523/125 , C12Q2531/125
摘要： The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods.
摘要翻译： 本发明提供了DNA的亚硫酸氢盐转化的改进方法，并且有助于分析基因组DNA的胞嘧啶甲基化。 相对于现有技术的方法，变性溶剂，新反应条件和新的纯化方法的新型组合为DNA的亚硫酸氢盐转化提供了惊人的有效方法。 随后可以通过许多不同的方法分析转化的DNA。
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公开(公告)号：US20090263810A1
公开(公告)日：2009-10-22
申请号：US12410389
申请日：2009-03-24
申请人： Kurt Berlin , Matthias Ballhause , Karen Cardon
发明人： Kurt Berlin , Matthias Ballhause , Karen Cardon
CPC分类号： C12Q1/6827 , C12Q2533/101 , Y10T436/143333 , C12Q2527/125 , C12Q2523/125 , C12Q2531/125
摘要： The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods.
摘要翻译： 本发明提供了DNA的亚硫酸氢盐转化的改进方法，并且有助于分析基因组DNA的胞嘧啶甲基化。 相对于现有技术的方法，变性溶剂，新反应条件和新的纯化方法的新型组合为DNA的亚硫酸氢盐转化提供了惊人的有效方法。 随后可以通过许多不同的方法分析转化的DNA。
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6.发明申请Methods and nucleic acids for the analysis of colorectal cell proliferative disorders 审中-公开用于分析结肠直肠细胞增殖性疾病的方法和核酸公开(公告)号：US20070184438A1
公开(公告)日：2007-08-09
申请号：US10562377
申请日：2004-06-23
申请人： Cathy Lofton-Day , Andrew Sledziewski , Jeff Thomas , Robert Day , Lori Tonnes-Priddy , Karen Cardon
发明人： Cathy Lofton-Day , Andrew Sledziewski , Jeff Thomas , Robert Day , Lori Tonnes-Priddy , Karen Cardon
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/154
摘要： Various aspects of the present invention provide novel diagnostic and prognostic methods for detecting, or for detecting and differentiating between or among colorectal cell proliferative disorders. Preferably, said colorectal cell proliferative disorders are selected from the group consisting of colorectal carcinoma, colon adenomas, and colon polyps. The inventive methods are based on analysis of differential CpG dinucleotide methylation of genomic DNA between or among normal and disease states. Additional embodiments provide nucleic acids and oligomers (including oligonucleotides and peptide nucleic acid (PNA)-oligomers), nucleic acid arrays and kits useful for practicing said methods, and in otherwise detecting, or detecting and differentiating between or among colorectal cell proliferative disorders.
摘要翻译： 本发明的各个方面提供用于检测或检测和分化结肠直肠细胞增殖性疾病之间或之间的新型诊断和预后方法。 优选地，所述结肠直肠细胞增殖性病症选自结肠直肠癌，结肠腺瘤和结肠息肉。 本发明的方法基于正常和疾病状态之间或之间基因组DNA差异CpG二核苷酸甲基化的分析。 另外的实施方案提供核酸和寡聚体（包括寡核苷酸和肽核酸（PNA）寡聚体）），用于实施所述方法的核酸阵列和试剂盒，以及另外检测或检测和分化结直肠细胞增殖性疾病之间或之间的核酸和寡核苷酸。
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