发明申请
- 专利标题： Dna amplification apparatus and method
- 专利标题（中）： Dna扩增装置及方法
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申请号： US10516186申请日： 2003-05-30
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公开(公告)号： US20060147912A1公开(公告)日： 2006-07-06
- 发明人: John Corbett , John Corbett
- 申请人： John Corbett , John Corbett
- 优先权： AUPS2678 20020530
- 国际申请： PCT/AU03/00672 WO 20030530
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34 ; C12M1/34
摘要：
The invention relates to a method for the amplification of DNA and apparatus for such amplification. The method comprises steps typically found in amplification methods but utilises an optical procedure to detect denaturation of DNA or attainment of the desired denaturation temperature. The apparatus (1) comprises a temperature controllable chamber (2) including a rotor (3) for holding a plurality of reaction vessels (see 7 for example) for reaction mixtures including DNA, a drive means for the rotor, a heater (8) within the chamber for transiently supplying infrared energy to the reaction vessels, and an optical system (12-16) for determining denaturation of at least a reference DNA or for detecting attainment of a desired denaturation temperature in at least a reference reaction vessel.
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