发明申请
- 专利标题: Methylotroph producing mammalian type sugar chain
- 专利标题(中): 生产甲基鱼油的哺乳动物类型的糖链
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申请号: US10511436申请日: 2003-04-28
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公开(公告)号: US20060148039A1公开(公告)日: 2006-07-06
- 发明人: Kazuo Kobayashi , Yoshinori Kitagawa , Toshihiro Komeda , Nagako Kawashima , Yoshifumi Jigami , Yasumori Chiba
- 申请人: Kazuo Kobayashi , Yoshinori Kitagawa , Toshihiro Komeda , Nagako Kawashima , Yoshifumi Jigami , Yasumori Chiba
- 优先权: JP2002-127677 20020426
- 国际申请: PCT/JP03/05464 WO 20030428
- 主分类号: C12P19/28
- IPC分类号: C12P19/28 ; C12N15/74 ; C12N1/18
摘要:
This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an α-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the α-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity. By introducing a mammalian type sugar chain biosynthesizing gene into the above-described mutant, a mammalian type sugar chain, such as a hybrid or complex, or a protein comprising a mammalian type sugar chain can be efficiently produced.
公开/授权文献
- US07972809B2 Methylotrophic yeast producing mammalian type sugar chain 公开/授权日:2011-07-05