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    Methylotroph producing mammalian type sugar chain
    1.
    发明申请
    Methylotroph producing mammalian type sugar chain 有权
    生产甲基鱼油的哺乳动物类型的糖链

    公开(公告)号：US20060148039A1

    公开(公告)日：2006-07-06

    申请号：US10511436

    申请日：2003-04-28

    申请人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasumori Chiba

    发明人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasumori Chiba

    IPC分类号： C12P19/28 ,  C12N15/74 ,  C12N1/18

    CPC分类号： C12P19/26 ,  C07K16/243 ,  C07K2317/21 ,  C12N1/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/2488 ,  C12N9/60 ,  C12N9/88 ,  C12N9/93 ,  C12P21/02 ,  C12Y101/01085 ,  C12Y101/03013 ,  C12Y102/01012 ,  C12Y302/01024 ,  C12Y402/01019 ,  C12Y603/02006

    摘要： This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an α-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the α-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity. By introducing a mammalian type sugar chain biosynthesizing gene into the above-described mutant, a mammalian type sugar chain, such as a hybrid or complex, or a protein comprising a mammalian type sugar chain can be efficiently produced.

    摘要翻译： 本发明提供一种生产包含哺乳动物型糖链的糖蛋白的方法，其特征在于该方法包括将α-1,2-甘露糖苷酶基因导入具有糖链生物合成酶基因突变的甲基营养酵母中，因此 α-1,2-甘露糖苷酶基因在酵母中有效启动子的控制下表达; 在介质中培养具有转移到其中的异源基因的甲基营养酵母细胞; 从培养物中获得包含哺乳动物型糖链的糖蛋白。 使用新制造的具有糖链突变的甲基营养酵母，可以大量生产与由哺乳动物细胞如人细胞产生的高甘露糖型糖链或包含这种中性糖链的糖蛋白相同的中性糖链 纯度高。 通过将哺乳动物型糖链生物合成基因导入到上述突变体中，可以有效地制备哺乳动物型糖链，例如杂合体或复合物，或包含哺乳动物型糖链的蛋白质。

    Methylotrophic yeast producing mammalian type sugar chain
    2.
    发明授权
    Methylotrophic yeast producing mammalian type sugar chain 有权
    甲基营养酵母产生哺乳动物型糖链

    公开(公告)号：US07972809B2

    公开(公告)日：2011-07-05

    申请号：US10511436

    申请日：2003-04-28

    申请人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasunori Chiba

    发明人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasunori Chiba

    IPC分类号： C12P21/00 ,  C12N15/01

    CPC分类号： C12P19/26 ,  C07K16/243 ,  C07K2317/21 ,  C12N1/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/2488 ,  C12N9/60 ,  C12N9/88 ,  C12N9/93 ,  C12P21/02 ,  C12Y101/01085 ,  C12Y101/03013 ,  C12Y102/01012 ,  C12Y302/01024 ,  C12Y402/01019 ,  C12Y603/02006

    摘要： This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an α-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the α-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity. By introducing a mammalian type sugar chain biosynthesizing gene into the above-described mutant, a mammalian type sugar chain, such as a hybrid or complex, or a protein comprising a mammalian type sugar chain can be efficiently produced.

    摘要翻译： 本发明提供一种生产包含哺乳动物型糖链的糖蛋白的方法，其特征在于该方法包括将α-1,2-甘露糖苷酶基因导入具有糖链生物合成酶基因突变的甲基营养酵母中，因此 α-1,2-甘露糖苷酶基因在酵母中有效启动子的控制下表达; 在介质中培养具有转移到其中的异源基因的甲基营养酵母细胞; 从培养物中获得包含哺乳动物型糖链的糖蛋白。 使用新制造的具有糖链突变的甲基营养酵母，可以大量生产与由哺乳动物细胞如人细胞产生的高甘露糖型糖链或包含这种中性糖链的糖蛋白相同的中性糖链 纯度高。 通过将哺乳动物型糖链生物合成基因导入到上述突变体中，可以有效地制备哺乳动物型糖链，例如杂合体或复合物，或包含哺乳动物型糖链的蛋白质。

    1-cycloalkenyltetrazolinones
    6.
    发明授权
    1-cycloalkenyltetrazolinones 失效
    1-环烯基四唑啉酮

    公开(公告)号：US5654257A

    公开(公告)日：1997-08-05

    申请号：US601748

    申请日：1996-02-15

    申请人： Toshio Goto ,  Yoshinori Kitagawa ,  Seishi Ito ,  Katsuhiko Shibuya ,  Tatsuya Yamaoka ,  Chieko Ueno ,  Yoshiko Kyo

    发明人： Toshio Goto ,  Yoshinori Kitagawa ,  Seishi Ito ,  Katsuhiko Shibuya ,  Tatsuya Yamaoka ,  Chieko Ueno ,  Yoshiko Kyo

    IPC分类号： C07D401/06 ,  A01N47/38 ,  C07D257/04 ,  C07D403/06 ,  A01N43/713

    CPC分类号： C07D257/04 ,  A01N47/38

    摘要： 1-cycloalkenyltetrazolinone derivatives of the following herbicidal formula ##STR1## R.sup.1 is cycloalkenyl which may optionally be substituted by halogen C.sub.1-4 alkyl, or bicycloalkenyl which may optionally be substituted by halogen or C.sub.1-4 alkyl, andR.sup.2 and R.sup.3 each independently is alkyl, cycloalkyl, phenyl (optionally substituted by a member selected from the group consisting of nitro, cyano, halogen, alkyl, haloalkyl, alkoxy, haloalkoxy and alkylthio), alkenyl and alkynyl,orR.sup.2 and R.sup.3 together with the N-atom to which they are bonded form, a 5- or 6-membered heterocyclic ring which may optionally contain a further hetero atom, said heterocyclic ring optionally being benzo-condensed, or a C-.sub.1-4 alkyl,and intermediates therefore of the formula ##STR2##

    摘要翻译： 以下除草式R 1的1-环烯基四唑啉酮衍生物是可任选被卤素C 1-4烷基取代的环烯基，或可任意被卤素或C 1-4烷基取代的双环烯基，R 2和R 3各自独立地为烷基 ，环烷基，苯基（任选被选自硝基，氰基，卤素，烷基，卤代烷基，烷氧基，卤代烷氧基和烷硫基的成员取代），烯基和炔基，或者R 2和R 3与它们的N-原子一起 键合形式，可任选地含有另外的杂原子的所述5或6元杂环，所述杂环任选地被苯并稠合，或C 1-4烷基，因此具有下式的中间体：