- 专利标题： Bacillus transformation, transformants and mutant libraries
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申请号： US11215675申请日： 2005-08-30
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公开(公告)号： US20060199222A1公开(公告)日： 2006-09-07
- 发明人: Maria Diaz-Torres , Edwin Lee , Thomas Morrison , Volker Schellenberger , Olga Selifonova
- 申请人： Maria Diaz-Torres , Edwin Lee , Thomas Morrison , Volker Schellenberger , Olga Selifonova
- 主分类号： C40B40/02
- IPC分类号： C40B40/02 ; C12N1/21 ; C12N15/74
摘要：
The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ.
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