- 专利标题： Modified polymerases for improved incorporation of nucleotide analogues
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申请号： US10571706申请日： 2004-09-10
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公开(公告)号： US20060240439A1公开(公告)日： 2006-10-26
- 发明人: Geoffrey Smith , David Bailey , Raquel Sanches , Harold Swerdlow , David Earnshaw
- 申请人： Geoffrey Smith , David Bailey , Raquel Sanches , Harold Swerdlow , David Earnshaw
- 优先权： GB0321306.3 20030911
- 国际申请： PCT/GB04/03891 WO 20040910
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C07H21/04 ; C12P21/06 ; C12P19/34 ; C12N9/22
摘要：
The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
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