发明申请
- 专利标题: Single chain antibody with cleavable linker
- 专利标题(中): 具有可切割连接子的单链抗体
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申请号: US11411436申请日: 2006-04-25
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公开(公告)号: US20060252096A1公开(公告)日: 2006-11-09
- 发明人: Dongxing Zha , Byung-Kwon Choi , Tillman Gerngross
- 申请人: Dongxing Zha , Byung-Kwon Choi , Tillman Gerngross
- 申请人地址: US NH Lebanon 03766
- 专利权人: GlycoFi, Inc.
- 当前专利权人: GlycoFi, Inc.
- 当前专利权人地址: US NH Lebanon 03766
- 主分类号: G01N33/53
- IPC分类号: G01N33/53 ; C07H21/04 ; C12P21/06 ; C12N1/18 ; C07K16/44 ; C12N15/74
摘要:
Disclosed are antibodies and methods for making antibodies with desired glycosylation and efficient production. Host cells transformed with a nucleic acid encoding a fusion protein comprising a signal sequence, light and heavy immunoglobulin chains, each comprising a variable region and a constant region and separated by a spacer peptide comprising at least one proteolytic cleavage site are cultured to express the nucleic acids and are cleaved by appropriate proteases to produce antibodies.