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16 条记录 (耗时 0.031 秒)

    Surface, anchored Fc-bait antibody display system
    4.
    发明授权
    Surface, anchored Fc-bait antibody display system 有权
    表面，锚定的Fc诱饵抗体展示系统

    公开(公告)号：US09365846B2

    公开(公告)日：2016-06-14

    申请号：US13990941

    申请日：2011-11-29

    申请人： Hussam Hisham Shaheen ,  Dongxing Zha

    发明人： Hussam Hisham Shaheen ,  Dongxing Zha

    IPC分类号： C40B40/10 ,  C12N15/10 ,  C07K16/00 ,  C07K16/32 ,  C07K16/40 ,  C07K16/46

    CPC分类号： C12N15/1037 ,  C07K16/00 ,  C07K16/32 ,  C07K16/40 ,  C07K16/46 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/35 ,  C07K2317/41 ,  C07K2317/52 ,  C40B40/10

    摘要： The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. A bait complexed with a monovalent antibody fragment can be expressed on the surface of the host cell wherein the fragment may be assayed for antigen binding while full antibody is simultaneously secreted from the host cell. Methods of using the system for identifying antibodies that bind specifically to an antigen of interest are also provided. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of use thereof.

    摘要翻译： 本发明部分地提供同时使用分泌物和显示模式的抗体显示系统。 与单价抗体片段复合的诱饵可以在宿主细胞的表面上表达，其中可以测定片段的抗原结合，同时从宿主细胞同时分泌全抗体。 还提供了使用该系统鉴定特异性结合感兴趣的抗原的抗体的方法。 还提供了可用于制备抗体展示系统的多肽，多核苷酸和宿主细胞及其使用方法。

    SURFACE, ANCHORED FC-BAIT ANTIBODY DISPLAY SYSTEM
    5.
    发明申请
    SURFACE, ANCHORED FC-BAIT ANTIBODY DISPLAY SYSTEM 有权
    表面，锚定的FC-BIT抗体显示系统

    公开(公告)号：US20140186334A1

    公开(公告)日：2014-07-03

    申请号：US13990941

    申请日：2011-11-29

    申请人： Dongxing Zha ,  Hussam Hisham Shaheen

    发明人： Dongxing Zha ,  Hussam Hisham Shaheen

    IPC分类号： C12N15/10 ,  C07K16/46

    CPC分类号： C12N15/1037 ,  C07K16/00 ,  C07K16/32 ,  C07K16/40 ,  C07K16/46 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/35 ,  C07K2317/41 ,  C07K2317/52 ,  C40B40/10

    摘要： The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. A bait complexed with a monovalent antibody fragment can be expressed on the surface of the host cell wherein the fragment may be assayed for antigen binding while full antibody is simultaneously secreted from the host cell. Methods of using the system for identifying antibodies that bind specifically to an antigen of interest are also provided. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of use thereof.

    摘要翻译： 本发明部分地提供同时使用分泌物和显示模式的抗体显示系统。 与单价抗体片段复合的诱饵可以在宿主细胞的表面上表达，其中可以测定片段的抗原结合，同时从宿主细胞同时分泌全抗体。 还提供了使用该系统鉴定特异性结合感兴趣的抗原的抗体的方法。 还提供了可用于制备抗体展示系统的多肽，多核苷酸和宿主细胞及其使用方法。

    METABOLIC ENGINEERING OF A GALACTOSE ASSIMILATION PATHWAY IN THE GLYCOENGINEERED YEAST PICHIA PASTORIS
    8.
    发明申请
    METABOLIC ENGINEERING OF A GALACTOSE ASSIMILATION PATHWAY IN THE GLYCOENGINEERED YEAST PICHIA PASTORIS 审中-公开
    葡萄糖生物化学烟草中糖基化途径的代谢工程

    公开(公告)号：US20120003695A1

    公开(公告)日：2012-01-05

    申请号：US13202002

    申请日：2010-02-24

    申请人： Robert C. Davidson ,  Piotr Bobrowicz ,  Dongxing Zha

    发明人： Robert C. Davidson ,  Piotr Bobrowicz ,  Dongxing Zha

    IPC分类号： C12P21/00 ,  C12N9/64 ,  C12N15/81 ,  C12N9/16 ,  C12N9/24 ,  C12N1/19 ,  C12N9/72

    CPC分类号： C12P21/005 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/41 ,  C12N1/16 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/90 ,  C12N15/815

    摘要： Lower eukaryotic cells such as Pichia pastoris that normally cannot use galactose as a carbon source but which have been genetically engineered according to the methods herein to use galactose as a sole source of carbon are described. The cells are genetically engineered to express several of the enzymes comprising the Leloir pathway. In particular, the cells are genetically engineered to express a galactokinase, a UDP-galactose-C4-epimerase, and a galactose-1-phosphate uridyltransferase, and optionally a galactose permease. In addition, a method is provided for improving the yield of glycoproteins that have galactose-terminated or -containing N-glycans in cells that have been genetically engineered to produce glycoproteins with N-glycans having galactose residues but which normally lack the enzymes comprising the Leloir pathway comprising transforming the cells with one or more nucleic acid molecules encoding a galactokinase, a UDP-galactose-C4-epimerase, and a galactose-1-phosphate uridyltransferase. The methods and host cells described enable the presence or lack of the ability to assimilate galactose as a selection method for making recombinant cells. The methods and host cells are shown herein to be particularly useful for making immunoglobulins and the like that have galactose-terminated or containing N-glycans.

    摘要翻译： 描述了通常不能使用半乳糖作为碳源但已经根据本文的方法进行遗传工程化的低等真核细胞，例如巴斯德毕赤酵母，其使用半乳糖作为唯一的碳源。 细胞被遗传工程化以表达几种包含Leloir途径的酶。 特别地，细胞被遗传工程化以表达半乳糖激酶，UDP-半乳糖-C4差向异构酶和半乳糖-1-磷酸尿苷酸转移酶，以及任选的半乳糖通透酶。 此外，提供了一种用于提高具有半乳糖封端或含有N-聚糖的糖蛋白的产率的方法，所述糖蛋白在已被遗传工程化以产生具有半乳糖残基的N-聚糖的糖蛋白的细胞中，但通常缺少包含Leloir的酶 途径包括用编码半乳糖激酶，UDP-半乳糖-C4-差向异构酶和半乳糖-1-磷酸尿苷转移酶的一种或多种核酸分子转化细胞。 所描述的方法和宿主细胞使得存在或缺乏同化半乳糖的能力作为制备重组细胞的选择方法。 本文显示的方法和宿主细胞特别可用于制备具有半乳糖终止或含有N-聚糖的免疫球蛋白等。

    SURFACE DISPLAY OF RECOMBINANT PROTEINS IN LOWER EUKARYOTES
    9.
    发明申请
    SURFACE DISPLAY OF RECOMBINANT PROTEINS IN LOWER EUKARYOTES 有权
    重组蛋白的表面显示在较低的毒素中

    公开(公告)号：US20100331192A1

    公开(公告)日：2010-12-30

    申请号：US12863911

    申请日：2009-02-20

    申请人： Dongxing Zha ,  Stefan Wildt

    发明人： Dongxing Zha ,  Stefan Wildt

    IPC分类号： C40B10/00 ,  G01N33/53

    CPC分类号： C12N15/1037 ,  C07K16/005 ,  C07K16/2887 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/51 ,  C07K2317/55 ,  C12N15/1055 ,  C40B40/02 ,  C40B40/08 ,  C40B50/06

    摘要： Methods for display of recombinant proteins or protein libraries on the surface of lower eukaryotes such as yeast and filamentous fungi are described. The methods are useful for screening libraries of recombinant proteins in lower eukaryotes to identify particular proteins with desired properties from the array of proteins in the libraries. The methods are particularly useful for constructing and screening antibody libraries in lower eukaryotes.

    摘要翻译： 描述了在低等真核生物如酵母和丝状真菌的表面上显示重组蛋白或蛋白质文库的方法。 该方法可用于筛选低等真核生物中的重组蛋白的文库，以从文库中的蛋白质阵列鉴定具有所需性质的特定蛋白质。 该方法对于构建和筛选低等真核生物中的抗体文库特别有用。