发明申请
US20060277628A1 Artificial promoter for the expression of dna sequences in vegetal cells
审中-公开
用于在植物细胞中表达dna序列的人工启动子
- 专利标题: Artificial promoter for the expression of dna sequences in vegetal cells
- 专利标题(中): 用于在植物细胞中表达dna序列的人工启动子
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申请号: US10539476申请日: 2003-12-19
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公开(公告)号: US20060277628A1公开(公告)日: 2006-12-07
- 发明人: Guillermo Sosa , Alberto Radriguez , Daymi Remedios , Osmany Gonzalez
- 申请人: Guillermo Sosa , Alberto Radriguez , Daymi Remedios , Osmany Gonzalez
- 申请人地址: CU Ciudad de La Habana
- 专利权人: CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA
- 当前专利权人: CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA
- 当前专利权人地址: CU Ciudad de La Habana
- 优先权: CU2002-0337 20021227
- 国际申请: PCT/CU03/00018 WO 20031219
- 主分类号: A01H5/00
- IPC分类号: A01H5/00 ; A01H1/00 ; C12N15/82 ; C12N5/04
摘要:
An artificial promoter characterized for been a chimerical recombinant DNA molecule such that, when introduced in any class of plant cells, promotes high expression levels of any DNA molecule fused to its 3′ end. The basic genetic elements of the molecule described here are: a core promoter with a consensus TATA box, followed by an Exon/Intron/Exon region and a translation enhancer element, all of them artificially constructed. Transcription regulatory elements can be inserted upstream of the promoter here described to confer temporal-, organ- or tissue-specificity to the expression. The designed artificial genetic elements can be functionally inserted between any promoter active in plant cells and any DNA sequence to increase its transcription/translation levels.
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