公开(公告)号：US20060277628A1

公开(公告)日：2006-12-07

申请号：US10539476

申请日：2003-12-19

申请人： Guillermo Sosa ,  Alberto Radriguez ,  Daymi Remedios ,  Osmany Gonzalez

发明人： Guillermo Sosa ,  Alberto Radriguez ,  Daymi Remedios ,  Osmany Gonzalez

IPC分类号： A01H5/00 ,  A01H1/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8216

摘要： An artificial promoter characterized for been a chimerical recombinant DNA molecule such that, when introduced in any class of plant cells, promotes high expression levels of any DNA molecule fused to its 3′ end. The basic genetic elements of the molecule described here are: a core promoter with a consensus TATA box, followed by an Exon/Intron/Exon region and a translation enhancer element, all of them artificially constructed. Transcription regulatory elements can be inserted upstream of the promoter here described to confer temporal-, organ- or tissue-specificity to the expression. The designed artificial genetic elements can be functionally inserted between any promoter active in plant cells and any DNA sequence to increase its transcription/translation levels.

摘要翻译： 一种人工启动子，其特征在于是嵌合重组DNA分子，使得当引入任何类别的植物细胞时，促进与其3'末端融合的任何DNA分子的高表达水平。 本文描述的分子的基本遗传元件是：具有共有TATA盒的核心启动子，其后是外显子/内含子/外显子区域和翻译增强子元件，所有这些都是人为构建的。 转录调节元件可以插入在这里描述的启动子的上游，以赋予表达的时间，组织或组织特异性。 设计的人造遗传元件可以功能性地插入到任何在植物细胞中活化的启动子和任何DNA序列之间以增加其转录/翻译水平。