发明申请
- 专利标题： Fluorogenic enzyme substrates and methods of preparation
- 专利标题（中）： 荧光酶底物及其制备方法
-
申请号： US10540057申请日： 2003-12-26
-
公开(公告)号： US20070037234A1公开(公告)日： 2007-02-15
- 发明人: Jan Goeman , Koenraad Van Acker , Johan Van Der Eycken , Inge Dierynck
- 申请人： Jan Goeman , Koenraad Van Acker , Johan Van Der Eycken , Inge Dierynck
- 优先权： EP02102898.0 20021227
- 国际申请： PCT/EP03/51105 WO 20031226
- 主分类号： C12Q1/66
- IPC分类号： C12Q1/66 ; C07D239/72
摘要：
This invention relates to hydrolase fluorogenic substrates with improved cell permeability, methods for the preparation thereof, and methods of measuring activities of hydrolases, particularly in cell-based assays. The substrates easily diffuse into the cells, where they are enzymatically processed to yield photostable fluorescent products, and are particularly fitted for visualising enzyme-derived activities in cell-based assays.
公开/授权文献
- US07863428B2 Fluorogenic enzyme substrates and methods of preparation 公开/授权日：2011-01-04