A fixture for testing a mid-plane of a blade server includes a base board, a fixture unit and a carrier enclosure. The fixture unit is fixed on the base board, the fixture unit includes a receiving location for mounting the mid-plane thereon. The carrier enclosure is slideably attached on the base board and docked with the mid-plane. The fixture for testing a mid-plane of a blade server uses two separated modules to assist to test the mid-plane, there is no need to remove all modules from the blade server, whereupon simplifies the attachment and removing mid-plane procedure.