发明申请
- 专利标题： DNA sequencing method using step by step reaction
- 专利标题（中）： DNA测序方法采用逐步反应
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申请号： US11356187申请日： 2006-02-17
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公开(公告)号： US20070054283A1公开(公告)日： 2007-03-08
- 发明人: Akihiko Kishimoto , Hideki Kambara , Tomoharu Kajiyama , Guohua Zhou
- 申请人： Akihiko Kishimoto , Hideki Kambara , Tomoharu Kajiyama , Guohua Zhou
- 专利权人： Hitachi, Ltd.
- 当前专利权人： Hitachi, Ltd.
- 优先权： JP2005-257926 20050906
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The present invention provides an inexpensive DNA sequencing method with high-sensitivity. The method of the present invention comprising the steps of, adding an given amount of dATP for step by step complementary strand synthesis and subtracting the background luminescence intensity caused by dATP from the measured luminescence intensity to obtain the luminescence intensity involved in complementary strand synthesis.