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114 条记录 (耗时 0.041 秒)

    DNA sequencing method using step by step reaction
    1.
    发明申请
    DNA sequencing method using step by step reaction 审中-公开
    DNA测序方法采用逐步反应

    公开(公告)号：US20070054283A1

    公开(公告)日：2007-03-08

    申请号：US11356187

    申请日：2006-02-17

    申请人： Akihiko Kishimoto ,  Hideki Kambara ,  Tomoharu Kajiyama ,  Guohua Zhou

    发明人： Akihiko Kishimoto ,  Hideki Kambara ,  Tomoharu Kajiyama ,  Guohua Zhou

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2537/149

    摘要： The present invention provides an inexpensive DNA sequencing method with high-sensitivity. The method of the present invention comprising the steps of, adding an given amount of dATP for step by step complementary strand synthesis and subtracting the background luminescence intensity caused by dATP from the measured luminescence intensity to obtain the luminescence intensity involved in complementary strand synthesis.

    摘要翻译： 本发明提供了具有高灵敏度的便宜的DNA测序方法。 本发明的方法包括以下步骤：加入给定量的dATP进行逐步互补链合成，并从测量的发光强度减去由dATP引起的背景发光强度，以获得互补链合成中所涉及的发光强度。

    Method and reagent for sequencing
    2.
    发明申请
    Method and reagent for sequencing 审中-公开
    测序方法和试剂

    公开(公告)号：US20070166729A1

    公开(公告)日：2007-07-19

    申请号：US11542246

    申请日：2006-10-04

    申请人： Hideki Kambara ,  Guohua Zhou ,  Tomoharu Kajiyama ,  Shigeya Suzuki

    发明人： Hideki Kambara ,  Guohua Zhou ,  Tomoharu Kajiyama ,  Shigeya Suzuki

    IPC分类号： C12Q1/68 ,  C12Q1/66

    CPC分类号： C12Q1/66 ,  C12Q1/6869 ,  C12Q2565/301

    摘要： The present invention provides: a method for nucleic acid analysis including the steps of subjecting a reaction solution containing a sample nucleic acid to complementary strand synthesis with the sample nucleic acid as a template, reacting pyrophosphate produced in the complementary strand synthesis with 30 to 800 μM AMP in the coexistence of pyruvate phosphate dikinase to produce ATP, performing luciferase reaction with the ATP as a reaction substrate, and detecting chemiluminescence generated in the luciferase reaction to determine the presence or absence of the complementary strand synthesis; and a kit therefor.

    摘要翻译： 本发明提供了一种核酸分析方法，包括以下步骤：将含有样品核酸的反应溶液与样品核酸作为模板进行互补链合成，使互补链合成中产生的焦磷酸与30〜800μm AMP在丙二酸磷酸二激酶共存产生ATP，与ATP作为反应底物进行荧光素酶反应，并检测在荧光素酶反应中产生的化学发光，以确定互补链合成的存在或不存在; 和一个工具包。

    Multiplex SNP typing by bioluminometric assay coupled with terminator incorporation
    3.
    发明申请
    Multiplex SNP typing by bioluminometric assay coupled with terminator incorporation 审中-公开
    通过生物光谱测定与终止子掺入进行复制SNP分型

    公开(公告)号：US20060240445A1

    公开(公告)日：2006-10-26

    申请号：US11209652

    申请日：2005-08-24

    申请人： Hideki Kambara ,  Guohua Zhou ,  Tomoharu Kajiyama

    发明人： Hideki Kambara ,  Guohua Zhou ,  Tomoharu Kajiyama

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6823 ,  C12Q1/6869 ,  C12Q2565/301 ,  C12Q2563/103 ,  C12Q2525/186

    摘要： The present invention provides a method for analyzing a nucleotide sequence comprising the steps of: carrying out complementary strand synthesis by adding at least one of four kinds of ddNTP corresponding to nucleotides A, G, T, and C, or derivatives thereof to a reaction vessel containing a nucleic acid sample to extend one nucleotide at a target site; performing a bioluminescent reaction with the use of ATP formed from released pyrophosphate as a reaction substrate; and typing the target site by determining the presence or absence of the complementary strand synthesis based on a result of the bioluminescent reaction. The method of the present invention allows multiplex SNPs to be typed in one reaction vessel

    摘要翻译： 本发明提供了分析核苷酸序列的方法，包括以下步骤：通过将对应于核苷酸A，G，T和C的四种ddNTP或其衍生物中的至少一种或其衍生物加入到反应容器中来进行互补链合成 含有核酸样品以在靶位点延伸一个核苷酸; 使用由释放的焦磷酸盐形成的ATP作为反应底物进行生物发光反应; 并通过基于生物发光反应的结果确定互补链合成的存在或不存在来分类靶位点。 本发明的方法允许在一个反应​​容器中输入多重SNP

    Nucleic acid amplification device
    4.
    发明申请
    Nucleic acid amplification device 有权
    核酸扩增装置

    公开(公告)号：US20090215162A1

    公开(公告)日：2009-08-27

    申请号：US12292167

    申请日：2008-11-13

    申请人： Tomoharu Kajiyama ,  Masataka Shirai ,  Hideki Kambara

    发明人： Tomoharu Kajiyama ,  Masataka Shirai ,  Hideki Kambara

    IPC分类号： C12M1/40

    CPC分类号： B01L3/50851 ,  B01L3/5025 ,  B01L3/50857 ,  B01L2200/0668 ,  B01L2300/0819

    摘要： This invention provides a nucleic acid amplification device whereby the abundance of a target molecule can be maintained before and after a step of separately amplifying a sample such that highly accurate analysis results that can be applied to gene expression analysis can be obtained. Also, a nucleic acid amplification device having a structure in which a plurality of minute reaction cells each comprising a set of a bead-retaining space capable of retaining a single analysis bead and a reagent reaction space retaining no bead but having a volume that is large enough to cause a reagent reaction therein are positioned so as to form a planar face is provided.

    摘要翻译： 本发明提供了一种核酸扩增装置，其可以在分别扩增样品的步骤之前和之后维持目标分子的丰度，使得可以获得可应用于基因表达分析的高精度分析结果。 另外，具有这样的结构的核酸扩增装置，其中多个微小反应单元各自包含一组能够保留单个分析珠的珠保留空间和不保留珠而具有大体积的试剂反应空间 足以使其中的试剂反应定位成形成平面。

    Large-scale parallel nucleic acid analysis method
    5.
    发明申请
    Large-scale parallel nucleic acid analysis method 审中-公开
    大规模并行核酸分析方法

    公开(公告)号：US20080318244A1

    公开(公告)日：2008-12-25

    申请号：US12213448

    申请日：2008-06-19

    申请人： Hiroko Matsunaga ,  Hideki Kambara ,  Tomoharu Kajiyama

    发明人： Hiroko Matsunaga ,  Hideki Kambara ,  Tomoharu Kajiyama

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6834 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2565/537

    摘要： It is intended to provide a technique for amplifying, individually and in parallel, nucleic acids contained in a mixture of plural kinds of nucleic acid samples. The present invention provides a nucleic acid analysis method comprising amplification means, whereby amplification reaction is performed in a reaction solution comprising a homogeneous solvent and comprising at least plural template nucleic acids and solid phase carriers comprising one or more kinds of amplification probes immobilized on the surface, to prevent amplified products attributed to two or more template nucleic acids from being replicated in one solid phase carrier. According to the present invention, plural kinds of analyte nucleic acid samples in a mixed state can be amplified individually and in parallel. This method achieves one solid phase carrier-one nucleic acid. Therefore, a higher density of solid phase carriers with obtained amplified products is easily achieved, leading to improved throughput of amplified product analysis. Reactions in all the amplification reaction steps are performed under homogeneous solvent conditions. Therefore, the method of the present invention is performed by convenient procedures and as such, is suitable to automation.

    摘要翻译： 旨在提供用于扩增多种核酸样品的混合物中包含的核酸的单独和并行扩增的技术。 本发明提供了包含扩增方法的核酸分析方法，其中扩增反应在包含均相溶剂并包含至少多个模板核酸的反应溶液中进行，并且包含固定在表面上的一种或多种扩增探针的固相载体 ，以防止归因于两种或更多种模板核酸的扩增产物被复制在一种固相载体中。 根据本发明，可以单独并行地扩增处于混合状态的多种分析物核酸样品。 该方法实现了一种固相载体一核酸。 因此，容易获得具有获得的扩增产物的较高密度的固相载体，从而提高扩增产物分析的产量。 所有扩增反应步骤中的反应在均相溶剂条件下进行。 因此，本发明的方法通过方便的程序进行，因此适用于自动化。