发明申请
- 专利标题: Nucleotide sequences which encode the pfk gene
- 专利标题(中): 编码pfk基因的核苷酸序列
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申请号: US10926293申请日: 2004-08-26
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公开(公告)号: US20070054379A1公开(公告)日: 2007-03-08
- 发明人: Bettina Mockel , Walter Pfefferle
- 申请人: Bettina Mockel , Walter Pfefferle
- 申请人地址: DE Dusseldorf
- 专利权人: Degussa AG
- 当前专利权人: Degussa AG
- 当前专利权人地址: DE Dusseldorf
- 优先权: DE19956131.1 19991123
- 主分类号: C12P13/08
- IPC分类号: C12P13/08 ; C12N9/10 ; C07H21/04 ; C12N15/74 ; C12N1/21
摘要:
The present invention is directed to nucleotide sequences coding for phosphofructokinase which have been Corynebacterium glutamicum, and a process for the production of an L-amino acid comprising culturing a coryneform bacteria comprising an overexpressed pfk gene, wherein said pfk gene comprises a polynucleotide having a nucleotide sequence which is at least 90% identical to the nucleotide of SEQ ID NO: 1 encoding a polypeptide having the enzymatic activity of a phosphofructokinase, accumulating said L-amino acid in the medium or in the cells of said bacterium, and isolated said L-amino acid.
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