Invention Application
US20070070349A1 Optical train and method for TIRF single molecule detection and analysis
审中-公开
光学列车和TIRF单分子检测与分析方法
- Patent Title: Optical train and method for TIRF single molecule detection and analysis
- Patent Title (中): 光学列车和TIRF单分子检测与分析方法
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Application No.: US11234420Application Date: 2005-09-23
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Publication No.: US20070070349A1Publication Date: 2007-03-29
- Inventor: Timothy Harris , Philip Buzby , Mirna Jarosz , Jaime Gill , Howard Weiss , Stanley Lapidus
- Applicant: Timothy Harris , Philip Buzby , Mirna Jarosz , Jaime Gill , Howard Weiss , Stanley Lapidus
- Applicant Address: US MA Cambridge
- Assignee: Helicos BioSciences Corporation
- Current Assignee: Helicos BioSciences Corporation
- Current Assignee Address: US MA Cambridge
- Main IPC: G01N21/64
- IPC: G01N21/64
Abstract:
In one aspect the invention relates to an apparatus for analyzing the presence of a single molecule using total internal reflection. In one embodiment an apparatus for single molecule analysis includes a support having a sample located thereon; two sources of light at distinct wavelengths, a collimator for directing the light onto the sample through a total internal reflection objective; a receiver for receiving a fluorescent emission produced by a single molecule in the sample in response to the light; and a detector for detecting each of the wavelengths in the fluorescent emission. In another embodiment the apparatus further comprises a focusing laser for maintaining focus of the objective on the sample.
Information query
