A cartridge includes a first layer to receive a printed circuit board hosting a biochip. A second layer is connected to the first layer and is configured for sample injection to the biochip. The second layer includes a first septum to isolate fluidic ports. A third layer is connected to the second layer and is configured for fluidic purge operations. The third layer includes a second septum to isolate a fluidic channel. A fourth layer is connected to the third layer and is configured to receive and route fluidic samples to the third layer.