A method provides flow to multiple channels in an endoscope during an endoscope decontamination procedure. A member is inserted into a chamber in a body of an endoscope through an opening into the chamber, the chamber being intersected by a first lumen and a second lumen. The member separates fluidly the first lumen from the second lumen. Fluid is flowed through a first channel through the member into the first lumen and through a second channel through the member into the second lumen. Additional lumens can be so treated at the same time. Fluids can include detergents and other cleaning fluids, disinfectants and sterilants.